Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31304
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-OCT-2001, sequence version 1.
07-MAR-2006, entry version 29.
Phylogenetic order:
Archaea Euryarchaeota Thermoplasmata Thermoplasmatales Thermoplasmataceae Thermoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_THEVO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000011 | BAB60143.1 | - |
| HSSP | P12638 | 1QUM | |
| GenomeReviews | BA000011_GR | TV1001.1 | |
| BioCyc | TVOL50339:TVN0971-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GSS1 / DSM 4299 / JCM 9571;
RX MEDLINE=20570466; PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA Kawashima T., Amano N., Koike H., Makino S., Higuchi S.,
RA Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T.,
RA Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT "Archaeal adaptation to higher temperatures revealed by genomic
RT sequence of Thermoplasma volcanium.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
Feature:
CHAIN 1 280 Probable endonuclease IV.
/FTId=PRO_0000190895.
METAL 77 77 Zinc 1 (By similarity).
METAL 117 117 Zinc 1 (By similarity).
METAL 148 148 Zinc 1 (By similarity).
METAL 148 148 Zinc 2 (By similarity).
METAL 180 180 Zinc 2 (By similarity).
METAL 183 183 Zinc 3 (By similarity).
METAL 215 215 Zinc 2 (By similarity).
METAL 228 228 Zinc 3 (By similarity).
METAL 230 230 Zinc 3 (By similarity).
METAL 259 259 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 280
MDDDLKSIAS KYYIGGHISV SGGLHLAPER ASIFGFRTFQ FFSKNQMRWN STPISEEEAL
RFISEVSSHS ISSTMVHASY LINLASSSNE LHQKSFNAFV EEIQRAERID ATFLTFHPGS
NGNKDEGIRK IKEAIEKVET HKVKLLVENT AGQGNVIGST IYEIGQIIDG FDSSVGVCID
TCHAWAAGYD ITNKYDEFID ELDSAIGLDR IYAFHLNDAM KDLGSNIDRH ELIGRGKIGE
GLIKLISDVR LFGKPKIMET PYGEAKFEEN LKYIRKKLGE