Description:
Bifunctional methyltransferase/endonuclease [Includes: Probablemethylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.-) (O-6-methylbase-DNA-alkyltransferase); Endonuclease V (EC 3.1.21.7)(Deoxyinosine 3'endonuclease) (Deoxyribonuclease V) (DNase V)].
Molecular weight: 36049
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
01-OCT-2001, sequence version 1.
07-MAR-2006, entry version 24.
Phylogenetic order:
Archaea Euryarchaeota Thermoplasmata Thermoplasmatales Thermoplasmataceae Thermoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein NFI_THEVO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000011 | BAB59937.1 | - |
| HSSP | O74023 | 1MGT | |
| GenomeReviews | BA000011_GR | TV0795.1 | |
| BioCyc | TVOL50339:TVN0781-MONOMER | -.1 | |
| HAMAP | MF_00801 | fused | 1. |
| InterPro | IPR007581 | Endonuc_V. | |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| Pfam | PF04493 | Endonuclease_5 | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Magnesium; Methyltransferase; Multifunctional enzyme; Nuclease; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GSS1 / DSM 4299 / JCM 9571;
RX MEDLINE=20570466; PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA Kawashima T., Amano N., Koike H., Makino S., Higuchi S.,
RA Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T.,
RA Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT "Archaeal adaptation to higher temperatures revealed by genomic
RT sequence of Thermoplasma volcanium.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
Feature:
CHAIN 1 321 Bifunctional
methyltransferase/endonuclease.
/FTId=PRO_0000159700.
REGION 1 86 Probable methylated-DNA--protein-cysteine
methyltransferase.
REGION 87 318 Endonuclease V.
ACT_SITE 61 61 Potential.
Comments:
-!- FUNCTION: Selectively cleaves double-stranded DNA at the second
phosphodiester bond 3' to a deoxyinosine leaving behind the intact
lesion on the nicked DNA. Acts in DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at apurinic or
apyrimidinic sites to products with a 5'-phosphate.
-!- COFACTOR: Magnesium (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: In the N-terminal section; belongs to the MGMT family.
-!- SIMILARITY: In the C-terminal section; belongs to the endonuclease
V family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 321
MLSVDYENFD LISYLYNLVR QIPDGMVSTY GDLAEALGDP VAARSVGFML SINKEPDYIP
CYKVVPHDGS VGNYTHPLGS AEKMRRLIRD GITITNGHIS NFEKVRFREF KTDYPLRKLR
ELQFKIGRLY DDRNDYSLDT IAAFDVSYKE TRGYASKVVY NKGKIGAYVY SSDSMFPYIP
GYLAFKEFKF IRALYDNETM ILIDGNGILH PRFAGLATHA GVSLKTASIG IAKHLINCTV
KGSDLLISGV VAGKMIGHHT IVSPGNRINV EEAGRLIEQR EGKEMRSLLR LAHNLTRLHI
ETNGNIARYD FSTRNTAIQS S