Description:
Probable thermostable DNA ligase (EC 6.5.1.1) (Polydeoxyribonucleotidesynthase [ATP]).
Molecular weight: 63623
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-AUG-1998, sequence version 1.
07-MAR-2006, entry version 40.
Phylogenetic order:
Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLI_PYRHO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000001 | BAA30734.1 | - |
| PIR | F71041 | F71041. | |
| GenomeReviews | BA000001_GR | PH1622.1 | |
| BioCyc | PHOR53953:PH1622-MONOMER | -.1 | |
| HAMAP | MF_00407 | - | 1. |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| InterPro | IPR012308 | DNA_ligase_A_N. | |
| InterPro | IPR012340 | OB_NA_bd_sub. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF04679 | DNA_ligase_A_C | 1. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| Pfam | PF04675 | DNA_ligase_A_N | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | FALSE_NEG. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
Keywords:
ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OT3;
RX MEDLINE=98344137; PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
Feature:
CHAIN 1 559 Probable thermostable DNA ligase.
/FTId=PRO_0000059614.
ACT_SITE 249 249 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein seals, during DNA replication, DNA
recombination and DNA repair, nicks in double-stranded DNA (By
similarity).
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- SUBUNIT: Monomer (By similarity).
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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Sequence length: 559
MKYIELAQLY QKLEKTTMKL IKTRLVADFL KRVPEDHLDF IPYLILGDVF PEWDERELGV
GEKLLIKAVS MATGVDPKEI ENSVKDTGDL GESIALAIKR RKQKSFFSQP LTIKRVYQTL
VKIAETTGEG SQEKKMKYLA NLFMDAEPLE AKYLARTVLG TMRTGVAEGL LRDAIALAFH
VKVELVERAY MLTSDFGFVA KIAKAEGNEG LEKVTIQLGK PIKPMLAQQA ANIKEALLEM
GGEAEFEIKY DGARVQVHKD GEKVIIYSRR LENVTRAIPE IVEAIKEAIE PEKVIVEGEL
VAIGEEGRPL PFQYVLRRFR RKHNIEEMMK KIPLELNLFD VLYVDGKSFV DTKFIERRKK
LEEIVKPNGK IKVAENLITK NAEEAEKFYK RALEMGHEGL MAKRLDAVYE PGNRGKKWLK
IKPTMENLDL VIIGAEWGEG RRAHLFGSFI LGAYDPETGE FLEVGKVGSG FTDDDLVEFT
KMLKPLIVKE EGKRVVIQPK IVIEVTYQEI QKSPKYRSGF ALRFPRYVAL RDDKGPEDAD
TIERIAQLYE LQERIKGKV