Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 19614
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) DNA dealkylation( GO:0006307 )
Important dates:
10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
10-MAY-2004, sequence version 2.
07-MAR-2006, entry version 29.
Phylogenetic order:
Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_PYRHO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000001 | BAA30955.1 | ALT_INIT |
| PIR | D71195 | D71195. | |
| HSSP | O74023 | 1MGT | |
| GenomeReviews | BA000001_GR | PH1835.1 | |
| BioCyc | PHOR53953:PH1835-MONOMER | -.1 | |
| HAMAP | MF_00772 | - | 1. |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OT3;
RX MEDLINE=98344137; PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
Feature:
CHAIN 1 172 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139384.
ACT_SITE 142 142 Alkyl group acceptor (By similarity).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated (By
similarity).
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 172
MLTYKTFKIL GREILIGVVW EEKIQGIAYS LDGLEFLKDQ LSRVTSHLKS RGVKVNLLEE
KSRYPDLVFD VLKGKIGNEK GFEELSLEGL TRFEIKVYSW LVKNVKRGEV ITYGKVAKAL
KTSPIAVGGA MKRNPYPIIV PCHRVVGKNN PWLYTPKPSY KKFLLEVEGW IS