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Description:
DNA-3-methyladenine glycosylase I (EC 3.2.2.20) (3-methyladenine-DNAglycosylase I, constitutive) (TAG I) (DNA-3-methyladenine glycosidaseI).
Molecular weight: 21100
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
13-AUG-1987, sequence version 1.
07-MAR-2006, entry version 53.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein 3MG1_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | J02606 | AAA24658.1 | - |
| EMBL | X03845 | CAA27472.1 | - |
| EMBL | U00039 | AAB18526.1 | - |
| EMBL | U00096 | AAC76573.1 | - |
| PIR | A24604 | DGECM1. | |
| PDB | 1LMZ | NMR | A=1-187. |
| PDB | 1NKU | NMR | A=1-187. |
| PDB | 1P7M | NMR | A=1-187. |
| ECO2DBASE | H021.1 | 6TH EDITION. | |
| GenomeReviews | U00096_GR | b3549.1 | |
| EchoBASE | EB0979 | -.1 | |
| EcoGene | EG10986 | tag. | |
| BioCyc | EcoCyc:EG10986-MONOMER | -.1 | |
| LinkHub | P05100 | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR005019 | Adenine_glyco. | |
| InterPro | IPR004597 | Tag. | |
| Pfam | PF03352 | Adenine_glyco | 1. |
| TIGRFAMs | TIGR00624 | tag | 1. |
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Hydrolase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-29.
RX MEDLINE=87057218; PubMed=3536912;
RA Sakumi K., Nakabeppu Y., Yamamoto Y., Kawabata S., Iwanaga S.,
RA Sekiguchi M.;
RT "Purification and structure of 3-methyladenine-DNA glycosylase I of
RT Escherichia coli.";
RL J. Biol. Chem. 261:15761-15766(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=86232617; PubMed=3520491;
RA Steinum A.-L., Seeberg E.;
RT "Nucleotide sequence of the tag gene from Escherichia coli.";
RL Nucleic Acids Res. 14:3763-3772(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=94316500; PubMed=8041620;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the
RT region from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
Feature:
CHAIN 1 187 DNA-3-methyladenine glycosylase I.
/FTId=PRO_0000194877.
STRAND 5 6
STRAND 9 10
TURN 11 11
HELIX 12 19
STRAND 20 23
HELIX 28 39
TURN 40 43
STRAND 44 44
HELIX 46 70
HELIX 73 81
STRAND 82 85
STRAND 89 89
HELIX 90 107
TURN 108 109
HELIX 112 118
TURN 119 122
STRAND 125 126
STRAND 130 131
TURN 132 134
STRAND 135 135
STRAND 137 137
HELIX 140 152
TURN 153 153
HELIX 159 169
TURN 170 170
STRAND 171 173
TURN 177 178
STRAND 179 181
Comments:
-!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to
excise 3-methyladenine from the damaged DNA polymer formed by
alkylation lesions.
-!- CATALYTIC ACTIVITY: Hydrolysis of alkylated DNA, releasing 3-
methyladenine.
-!- ENZYME REGULATION: Activity is controlled by product inhibition.
-!- INTERACTION:
P06958:aceE; NbExp=1; IntAct=EBI-558722, EBI-542683;
P0A7M6:rpmC; NbExp=1; IntAct=EBI-558722, EBI-546493;
P0A7W1:rpsE; NbExp=1; IntAct=EBI-558722, EBI-543949;
P0A8L1:serS; NbExp=1; IntAct=EBI-558722, EBI-548422;
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Sequence length: 187
MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL KKRENYRACF
HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR AYLQMEQNGE PFVDFVWSFV
NHQPQVTQAT TLSEIPTSTS ASDALSKALK KRGFKFVGTT ICYSFMQACG LVNDHVVGCC
CYPGNKP