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Description:
Alkylated DNA repair protein alkB.
Molecular weight: 24076
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
13-AUG-1987, sequence version 1.
07-MAR-2006, entry version 51.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ALKB_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | J02607 | AAA23416.1 | - |
| EMBL | M10315 | AAA23414.1 | - |
| EMBL | U00008 | AAA16409.1 | - |
| EMBL | U00096 | AAC75272.1 | - |
| EMBL | D90850 | BAA15995.1 | - |
| PIR | A24605 | BVECKB. | |
| GenomeReviews | U00096_GR | b2212.1 | |
| EchoBASE | EB0036 | -.1 | |
| EcoGene | EG10037 | alkB. | |
| BioCyc | EcoCyc:EG10037-MONOMER | -.1 | |
| LinkHub | P05050 | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR005123 | 2OG-FeII_Oase. | |
| InterPro | IPR004574 | Alkb. | |
| Pfam | PF03171 | 2OG-FeII_Oxy | 1. |
| TIGRFAMs | TIGR00568 | alkb | 1. |
Keywords:
Complete proteome; Direct protein sequencing; DNA damage; DNA repair.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-30.
RX MEDLINE=87057220; PubMed=3536913;
RA Kondo H., Nakabeppu Y., Kataoka H., Kuhara S., Kawabata S.,
RA Sekiguchi M.;
RT "Structure and expression of the alkB gene of Escherichia coli related
RT to the repair of alkylated DNA.";
RL J. Biol. Chem. 261:15772-15777(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
RC STRAIN=B;
RX MEDLINE=85190562; PubMed=3887409;
RA Demple B., Sedgwick B., Robins P., Totty N., Waterfield M.D.,
RA Lindahl T.;
RT "Active site and complete sequence of the suicidal methyltransferase
RT that counters alkylation mutagenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:2688-2692(1985).
RN [6]
RP CHARACTERIZATION.
RX MEDLINE=95014070; PubMed=7928996;
RA Chen B.J., Carroll P., Samson L.;
RT "The Escherichia coli AlkB protein protects human cells against
RT alkylation-induced toxicity.";
RL J. Bacteriol. 176:6255-6261(1994).
Feature:
CHAIN 1 216 Alkylated DNA repair protein alkB.
/FTId=PRO_0000066665.
Comments:
-!- FUNCTION: Probably involved in the repair of alkylated DNA.
Provides extensive resistance to alkylating agents such as MMS and
DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).
-!- INTERACTION:
P45476:yhcC; NbExp=1; IntAct=EBI-561190, EBI-555589;
-!- SIMILARITY: Belongs to the alkB family.
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Sequence length: 216
MLDLFADAEP WQEPLAAGAV ILRRFAFNAA EQLIRDINDV ASQSPFRQMV TPGGYTMSVA
MTNCGHLGWT THRQGYLYSP IDPQTNKPWP AMPQSFHNLC QRAATAAGYP DFQPDACLIN
RYAPGAKLSL HQDKDEPDLR APIVSVSLGL PAIFQFGGLK RNDPLKRLLL EHGDVVVWGG
ESRLFYHGIQ PLKAGFHPLT IDCRYNLTFR QAGKKE