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Description:
Excinuclease cho (EC 3.1.25.-) (Endonuclease cho) (UvrC homologprotein).
Molecular weight: 33739
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
16-APR-2002, integrated into UniProtKB/Swiss-Prot.
01-FEB-1997, sequence version 1.
07-MAR-2006, entry version 43.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein CHO_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U00096 | AAC74811.1 | - |
| PIR | E64933 | E64933. | |
| GenomeReviews | U00096_GR | b1741.1 | |
| EchoBASE | EB3749 | -.1 | |
| EcoGene | EG13993 | cho. | |
| BioCyc | EcoCyc:G6937-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR000305 | UvrC_N. | |
| Pfam | PF01541 | GIY-YIG | 1. |
| SMART | SM00465 | GIYc | 1. |
| PROSITE | PS50164 | UVRC_1 | 1. |
Keywords:
Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Hydrolase; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [2]
RP FUNCTION.
RX MEDLINE=21819418; PubMed=11818552; DOI=10.1073/pnas.032584099;
RA Moolenaar G.F., van Rossum-Fikkert S., van Kesteren M., Goosen N.;
RT "Cho, a second endonuclease involved in Escherichia coli nucleotide
RT excision repair.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:1467-1472(2002).
Feature:
CHAIN 1 295 Excinuclease cho.
/FTId=PRO_0000138370.
Comments:
-!- FUNCTION: Incises the DNA at the 3' side of a lesion during
nucleotide excision repair. Incises the DNA farther away from the
lesion than uvrC. Not able to incise the 5' site of a lesion. In
vitro, the incision activity of cho is uvrA and uvrB dependent.
When a lesion remains because uvrC is not able to induce the 3'
incision, cho incises the DNA. Then uvrC makes the 5' incision.
The combined action of cho and uvrC broadens the substrate range
of nucleotide excision repair.
-!- INTERACTION:
P0A6F5:groL; NbExp=1; IntAct=EBI-545155, EBI-543750;
-!- INDUCTION: Repressed by lexA.
-!- SIMILARITY: Contains 1 uvrC C-terminal domain.
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Sequence length: 295
MVRRLTSPRL EFEAAAIYEY PEHLRSFLND LPTRPGVYLF HGESDTMPLY IGKSVNIRSR
VLSHLRTPDE AAMLRQSRRI SWICTAGEIG ALLLEARLIK EQQPLFNKRL RRNRQLCALQ
LNEKRVDVVY AKEVDFSRAP NLFGLFANRR AALQALQTIA DEQKLCYGLL GLEPLSRGRA
CFRSALKRCA GACCGKESHE EHALRLRQSL ERLRVVCWPW QGAVALKEQH PEMTQYHIIQ
NWLWLGAVNS LEEATTLIRT PAGFDHDGYK ILCKPLLSGN YEITELDPAN DQRAS