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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 73606
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-APR-1990, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 67.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M30255 | AAA24071.1 | - |
| EMBL | M24278 | AAA24070.1 | - |
| EMBL | U00096 | AAC75464.1 | - |
| EMBL | D90870 | BAA16283.1 | ALT_FRAME |
| EMBL | D90870 | BAA16282.1 | ALT_FRAME |
| EMBL | U74650 | AAB42062.1 | - |
| PIR | B65015 | LQECC6. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | U00096_GR | b2411.1 | |
| EchoBASE | EB0529 | -.1 | |
| EcoGene | EG10534 | ligA. | |
| BioCyc | EcoCyc:EG10534-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| Pfam | PF00633 | HHH | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00292 | BRCT | 1. |
| SMART | SM00278 | HhH1 | 2. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | 1. |
| PROSITE | PS01055 | DNA_LIGASE_N1 | 1. |
| PROSITE | PS01056 | DNA_LIGASE_N2 | 1. |
Keywords:
Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-13 AND
RP 666-671.
RC STRAIN=K12 / C600;
RX MEDLINE=86310292; PubMed=3018436; DOI=10.1007/BF00330179;
RA Ishino Y., Shinagawa H., Makino K., Tsunasawa S., Sakiyama F.,
RA Nakata A.;
RT "Nucleotide sequence of the lig gene and primary structure of DNA
RT ligase of Escherichia coli.";
RL Mol. Gen. Genet. 204:1-7(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA O'Connor M.J., Ally A., Ally D., Zhang X., Robichaud M., Backman K.;
RL Submitted (APR-1989) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97349980; PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K.,
RA Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N.,
RA Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H.,
RA Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S.,
RA Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C.,
RA Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-
RT K12 genome corresponding to 50.0-68.8 min on the linkage map and
RT analysis of its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-90.
RC STRAIN=PB103;
RX MEDLINE=97160838; PubMed=9008158; DOI=10.1016/S0092-8674(00)81838-3;
RA Hale C.A., de Boer P.A.J.;
RT "Direct binding of FtsZ to ZipA, an essential component of the septal
RT ring structure that mediates cell division in E. coli.";
RL Cell 88:175-185(1997).
Feature:
CHAIN 1 671 DNA ligase.
/FTId=PRO_0000161745.
DOMAIN 593 671 BRCT.
ACT_SITE 115 115 N6-AMP-lysine intermediate (By
similarity).
CONFLICT 69 69 A -> R (in Ref. 1).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA.
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- INTERACTION:
P06958:aceE; NbExp=1; IntAct=EBI-553496, EBI-542683;
P07813:leuS; NbExp=1; IntAct=EBI-553496, EBI-553345;
P21645:lpxD; NbExp=1; IntAct=EBI-553496, EBI-542924;
P16926:mreC; NbExp=1; IntAct=EBI-553496, EBI-553515;
P36767:rdgC; NbExp=1; IntAct=EBI-553496, EBI-561716;
P15032:recE; NbExp=1; IntAct=EBI-553496, EBI-553304;
P0A7K2:rplL; NbExp=1; IntAct=EBI-553496, EBI-543702;
P0A7V0:rpsB; NbExp=1; IntAct=EBI-553496, EBI-543439;
P77212:ykgC; NbExp=1; IntAct=EBI-553496, EBI-553523;
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
-!- CAUTION: Ref.4 sequence differs from that shown due to frameshifts
in positions 289 and 313.
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Sequence length: 671
MESIEQQLTE LRTTLRHHEY LYHVMDAPEI PDAEYDRLMR ELRELETKHP ELITPDSPTQ
RVGAAPLAAF SQIRHEVPML SLDNVFDEES FLAFNKRVQD RLKNNEKVTW CCELKLDGLA
VSILYENGVL VSAATRGDGT TGEDITSNVR TIRAIPLKLH GENIPARLEV RGEVFLPQAG
FEKINEDARR TGGKVFANPR NAAAGSLRQL DPRITAKRPL TFFCYGVGVL EGGELPDTHL
GRLLQFKKWG LPVSDRVTLC ESAEEVLAFY HKVEEDRPTL GFDIDGVVIK VNSLAQQEQL
GFVARAPRWA VAFKFPAQEQ MTFVRDVEFQ VGRTGAITPV ARLEPVHVAG VLVSNATLHN
ADEIERLGLR IGDKVVIRRA GDVIPQVVNV VLSERPEDTR EVVFPTHCPV CGSDVERVEG
EAVARCTGGL ICGAQRKESL KHFVSRRAMD VDGMGDKIID QLVEKEYVHT PADLFKLTAG
KLTGLERMGP KSAQNVVNAL EKAKETTFAR FLYALGIREV GEATAAGLAA YFGTLEALEA
ASIEELQKVP DVGIVVASHV HNFFAEESNR NVISELLAEG VHWPAPIVIN AEEIDSPFAG
KTVVLTGSLS QMSRDDAKAR LVELGAKVAG SVSKKTDLVI AGEAAGSKLA KAQELGIEVI
DEAEMLRLLG S