|
|
|
|
|
|
Description:
Exodeoxyribonuclease I (EC 3.1.11.1) (Exonuclease I) (DNAdeoxyribophosphodiesterase) (dRPase).
Molecular weight: 54501
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
01-FEB-1994, sequence version 2.
07-MAR-2006, entry version 64.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EX1_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | J02641 | AAA19938.1 | - |
| EMBL | U00009 | AAA16417.1 | - |
| EMBL | U00096 | AAC75072.1 | - |
| EMBL | D90839 | BAA15839.1 | - |
| EMBL | D90840 | BAA15844.1 | - |
| PIR | B64966 | NCECX1. | |
| PDB | 1FXX | X-ray | A=1-475. |
| GenomeReviews | U00096_GR | b2011.1 | |
| EchoBASE | EB0919 | -.1 | |
| EcoGene | EG10926 | sbcB. | |
| BioCyc | EcoCyc:EG10926-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR006055 | Exonuclease. | |
| InterPro | IPR012337 | RNaseH_fold. | |
| Pfam | PF00929 | Exonuc_X-T | 1. |
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Exonuclease; Hydrolase; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-12.
RC STRAIN=K12;
RX MEDLINE=87083489; PubMed=3539937;
RA Phillips G.J., Kushner S.R.;
RT "Determination of the nucleotide sequence for the exonuclease I
RT structural gene (sbcB) of Escherichia coli K12.";
RL J. Biol. Chem. 262:455-459(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [5]
RP FUNCTION AS A DRPASE.
RX MEDLINE=93027182; PubMed=1329027;
RA Sandigursky M., Franklin W.A.;
RT "DNA deoxyribophosphodiesterase of Escherichia coli is associated with
RT exonuclease I.";
RL Nucleic Acids Res. 20:4699-4703(1992).
Feature:
CHAIN 1 475 Exodeoxyribonuclease I.
/FTId=PRO_0000087110.
CONFLICT 210 218 Missing (in Ref. 1).
CONFLICT 225 225 Q -> H (in Ref. 1).
CONFLICT 343 343 V -> E (in Ref. 1).
CONFLICT 475 475 V -> A (in Ref. 1).
STRAND 10 21
TURN 23 25
STRAND 26 26
STRAND 28 36
TURN 38 39
STRAND 42 42
STRAND 44 44
STRAND 47 51
TURN 55 56
HELIX 61 67
HELIX 71 77
STRAND 79 79
HELIX 81 92
TURN 93 93
STRAND 94 94
TURN 95 96
STRAND 97 103
TURN 104 107
HELIX 108 118
TURN 119 120
STRAND 121 121
STRAND 123 124
HELIX 125 127
TURN 128 128
STRAND 129 129
TURN 130 131
STRAND 133 136
HELIX 137 147
TURN 150 151
STRAND 152 152
HELIX 166 171
TURN 172 174
STRAND 179 179
TURN 180 181
STRAND 182 182
HELIX 183 200
HELIX 202 210
TURN 211 211
STRAND 213 213
HELIX 214 220
TURN 223 226
STRAND 229 232
HELIX 234 236
HELIX 238 240
TURN 241 242
STRAND 243 251
STRAND 253 254
TURN 256 257
STRAND 258 263
TURN 264 265
STRAND 266 266
HELIX 269 273
STRAND 274 274
HELIX 276 285
STRAND 286 286
STRAND 288 288
STRAND 298 302
TURN 303 304
STRAND 305 305
STRAND 308 311
HELIX 312 314
STRAND 315 315
HELIX 317 323
TURN 324 324
HELIX 327 339
TURN 341 341
HELIX 342 351
TURN 352 352
STRAND 361 361
HELIX 363 365
HELIX 367 369
HELIX 374 385
STRAND 386 386
HELIX 388 390
TURN 391 393
STRAND 400 400
TURN 401 401
HELIX 402 414
HELIX 416 418
HELIX 421 434
STRAND 435 435
HELIX 437 453
TURN 454 454
STRAND 455 456
Comments:
-!- FUNCTION: Also functions as a DNA deoxyribophosphodiesterase that
release deoxyribose-phosphate moieties following the cleavage DNA
at an apurinic/apyrimidinic (AP) site by either an AP endonuclease
AP lyase.
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage in the 3'- to 5'-
direction to yield nucleoside 5'-phosphates.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 475
MMNDGKQQST FLFHDYETFG THPALDRPAQ FAAIRTDSEF NVIGEPEVFY CKPADDYLPQ
PGAVLITGIT PQEARAKGEN EAAFAARIHS LFTVPKTCIL GYNNVRFDDE VTRNIFYRNF
YDPYAWSWQH DNSRWDLLDV MRACYALRPE GINWPENDDG LPSFRLEHLT KANGIEHSNA
HDAMADVYAT IAMAKLVKTR QPRLFDYLFT HRNKHKLMAL IDVPQMKPLV HVSGMFGAWR
GNTSWVAPLA WHPENRNAVI MVDLAGDISP LLELDSDTLR ERLYTAKTDL GDNAAVPVKL
VHINKCPVLA QANTLRPEDA DRLGINRQHC LDNLKILREN PQVREKVVAI FAEAEPFTPS
DNVDAQLYNG FFSDADRAAM KIVLETEPRN LPALDITFVD KRIEKLLFNY RARNFPGTLD
YAEQQRWLEH RRQVFTPEFL QGYADELQML VQQYADDKEK VALLKALWQY AEEIV