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Description:
Exodeoxyribonuclease III (EC 3.1.11.2) (Exonuclease III) (EXO III) (APendonuclease VI).
Molecular weight: 30969
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 4.
07-MAR-2006, entry version 71.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EX3_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X13002 | CAA31424.1 | - |
| EMBL | M22592 | AAA24767.1 | - |
| EMBL | U00096 | AAC74819.1 | - |
| EMBL | D90818 | BAA15540.1 | - |
| EMBL | D90819 | BAA15544.1 | - |
| PIR | E64934 | NCECX3. | |
| PDB | 1AKO | X-ray | @=1-268. |
| SWISS-2DPAGE | P09030 | COLI. | |
| ECO2DBASE | G028.2 | 6TH EDITION. | |
| GenomeReviews | U00096_GR | b1749.1 | |
| EchoBASE | EB1066 | -.1 | |
| EcoGene | EG11073 | xthA. | |
| BioCyc | EcoCyc:EG11073-MONOMER | -.1 | |
| LinkHub | P09030 | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR000097 | APendonclse1. | |
| InterPro | IPR005135 | Exo_endo_phos. | |
| InterPro | IPR004808 | ExoIII_xth. | |
| Pfam | PF03372 | Exo_endo_phos | 1. |
| TIGRFAMs | TIGR00195 | exoDNase_III | 1. |
| TIGRFAMs | TIGR00633 | xth | 1. |
| PROSITE | PS00726 | AP_NUCLEASE_F1_1 | 1. |
| PROSITE | PS00727 | AP_NUCLEASE_F1_2 | 1. |
| PROSITE | PS00728 | AP_NUCLEASE_F1_3 | 1. |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; Exonuclease; Hydrolase; Magnesium; Metal-binding; Multifunctional enzyme; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=89008066; PubMed=3049539;
RA Saporito S.M., Smith-White B.J., Cunningham R.P.;
RT "Nucleotide sequence of the xth gene of Escherichia coli K-12.";
RL J. Bacteriol. 170:4542-4547(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Wurst H., Hoheisel J.D., Pohl F.M.;
RL Submitted (NOV-1988) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251357; PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
RA Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
RA Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
RA Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
RA Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RX MEDLINE=95191690; PubMed=7885481; DOI=10.1038/374381a0;
RA Mol C.D., Kuo C.-F., Thayer M.M., Cunningham R.P., Tainer J.A.;
RT "Structure and function of the multifunctional DNA-repair enzyme
RT exonuclease III.";
RL Nature 374:381-386(1995).
RN [6]
RP CHARACTERIZATION.
RX MEDLINE=97105903; PubMed=8948651; DOI=10.1093/nar/24.22.4572;
RA Shida T., Noda M., Sekiguchi J.;
RT "Cleavage of single- and double-stranded DNAs containing an abasic
RT residue by Escherichia coli exonuclease III (AP endonuclease VI).";
RL Nucleic Acids Res. 24:4572-4576(1996).
Feature:
CHAIN 1 268 Exodeoxyribonuclease III.
/FTId=PRO_0000200021.
ACT_SITE 259 259 Proton acceptor.
METAL 34 34 Magnesium or manganese.
SITE 153 153 Important for substrate recognition.
CONFLICT 49 50 KL -> NV (in Ref. 1).
STRAND 2 7
STRAND 9 9
HELIX 11 13
HELIX 15 25
STRAND 28 33
HELIX 39 41
HELIX 44 49
TURN 50 51
STRAND 53 59
TURN 60 61
STRAND 62 71
STRAND 74 80
TURN 81 82
HELIX 85 88
TURN 89 90
STRAND 91 98
TURN 99 100
STRAND 101 109
STRAND 115 115
TURN 116 117
TURN 119 120
HELIX 121 139
TURN 142 143
STRAND 144 144
STRAND 146 151
STRAND 157 157
HELIX 158 160
STRAND 161 161
STRAND 163 163
HELIX 165 174
TURN 175 175
STRAND 176 179
HELIX 181 192
TURN 193 194
STRAND 195 197
HELIX 198 202
STRAND 203 203
TURN 204 205
STRAND 208 208
STRAND 211 212
TURN 215 218
HELIX 219 222
TURN 223 223
STRAND 226 226
STRAND 229 234
HELIX 235 238
TURN 239 240
STRAND 241 246
HELIX 248 251
TURN 252 252
STRAND 253 255
STRAND 258 259
STRAND 262 266
Comments:
-!- FUNCTION: Major apurinic-apyrimidinic endonuclease of E.coli. It
removes the damaged DNA at cytosines and guanines by cleaving on
the 3' side of the AP site by a beta-elimination reaction. It
exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair
diesterase and ribonuclease H activities.
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage in the 3'- to 5'-
direction to yield nucleoside 5'-phosphates.
-!- SUBUNIT: Monomer.
-!- INTERACTION:
P06958:aceE; NbExp=1; IntAct=EBI-559719, EBI-542683;
P06959:aceF; NbExp=1; IntAct=EBI-559719, EBI-542707;
P00391:lpdA; NbExp=1; IntAct=EBI-559719, EBI-542856;
P00490:malP; NbExp=1; IntAct=EBI-559719, EBI-551914;
-!- SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family.
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Sequence length: 268
MKFVSFNING LRARPHQLEA IVEKHQPDVI GLQETKVHDD MFPLEEVAKL GYNVFYHGQK
GHYGVALLTK ETPIAVRRGF PGDDEEAQRR IIMAEIPSLL GNVTVINGYF PQGESRDHPI
KFPAKAQFYQ NLQNYLETEL KRDNPVLIMG DMNISPTDLD IGIGEENRKR WLRTGKCSFL
PEEREWMDRL MSWGLVDTFR HANPQTADRF SWFDYRSKGF DDNRGLRIDL LLASQPLAEC
CVETGIDYEI RSMEKPSDHA PVWATFRR