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Description:
Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) (ExodeoxyribonucleaseV 67 kDa polypeptide).
Molecular weight: 66902
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 52.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EX5A_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X04582 | CAA28253.1 | - |
| EMBL | U29581 | AAB40466.1 | - |
| EMBL | U00096 | AAC75858.1 | - |
| EMBL | X04581 | CAA28251.1 | - |
| PIR | D65064 | NCECXF. | |
| PDB | 1W36 | X-ray | D/G=1-608. |
| GenomeReviews | U00096_GR | b2819.1 | |
| EchoBASE | EB0819 | -.1 | |
| EcoGene | EG10826 | recD. | |
| BioCyc | EcoCyc:EG10826-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR003593 | AAA_ATPase. | |
| InterPro | IPR006344 | RecD. | |
| InterPro | IPR000606 | Viral_helicase1. | |
| Pfam | PF01443 | Viral_helicase1 | 1. |
| SMART | SM00382 | AAA | 1. |
| TIGRFAMs | TIGR01447 | recD | 1. |
Keywords:
3D-structure; ATP-binding; Complete proteome; DNA damage; DNA repair; Endonuclease; Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=87066730; PubMed=3537961;
RA Finch P.W., Storey A., Brown K., Hickson I.D., Emmerson P.T.;
RT "Complete nucleotide sequence of recD, the structural gene for the
RT alpha subunit of Exonuclease V of Escherichia coli.";
RL Nucleic Acids Res. 14:8583-8594(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
RX MEDLINE=87066729; PubMed=3537960;
RA Finch P.W., Storey A., Chapman K.E., Brown K., Hickson I.D.,
RA Emmerson P.T.;
RT "Complete nucleotide sequence of the Escherichia coli recB gene.";
RL Nucleic Acids Res. 14:8573-8582(1986).
Feature:
CHAIN 1 608 Exodeoxyribonuclease V alpha chain.
/FTId=PRO_0000087115.
NP_BIND 171 178 ATP (Probable).
CONFLICT 247 248 QP -> HR (in Ref. 1).
CONFLICT 306 306 A -> V (in Ref. 1).
CONFLICT 390 390 F -> L (in Ref. 1).
TURN 3 3
HELIX 4 12
TURN 13 14
STRAND 15 16
HELIX 18 27
STRAND 28 31
HELIX 33 47
TURN 48 49
STRAND 51 54
TURN 55 60
HELIX 61 64
STRAND 65 67
STRAND 72 73
TURN 76 77
STRAND 79 79
TURN 80 80
HELIX 81 87
STRAND 88 88
TURN 89 90
STRAND 91 96
STRAND 99 102
STRAND 104 109
HELIX 110 123
TURN 124 124
STRAND 125 125
STRAND 128 128
HELIX 134 142
TURN 143 144
TURN 148 149
STRAND 150 150
HELIX 153 162
TURN 163 163
STRAND 164 170
TURN 173 174
STRAND 175 175
TURN 176 176
HELIX 177 190
TURN 191 191
STRAND 193 194
STRAND 199 205
HELIX 206 216
TURN 217 217
HELIX 218 222
STRAND 223 224
STRAND 227 227
STRAND 232 232
STRAND 234 234
STRAND 238 238
TURN 239 241
STRAND 242 242
TURN 257 258
STRAND 259 259
STRAND 262 262
STRAND 264 268
STRAND 270 270
HELIX 271 273
STRAND 275 275
HELIX 276 284
TURN 285 285
TURN 288 289
STRAND 291 296
TURN 298 299
STRAND 300 300
HELIX 301 303
TURN 304 304
STRAND 305 305
TURN 306 307
STRAND 308 308
HELIX 310 317
TURN 318 319
HELIX 323 332
STRAND 333 335
STRAND 341 341
TURN 344 345
HELIX 346 349
TURN 350 351
STRAND 352 354
STRAND 361 362
TURN 365 366
HELIX 367 374
TURN 375 375
STRAND 376 376
HELIX 378 382
TURN 383 385
STRAND 386 386
HELIX 387 389
STRAND 392 394
STRAND 397 397
STRAND 400 400
TURN 401 403
HELIX 404 414
TURN 415 415
HELIX 416 423
TURN 424 424
STRAND 428 429
TURN 430 431
HELIX 432 435
TURN 436 437
STRAND 438 443
STRAND 445 450
HELIX 451 462
TURN 463 463
STRAND 464 464
STRAND 525 525
STRAND 529 529
STRAND 531 532
STRAND 534 537
TURN 538 543
STRAND 546 552
STRAND 555 556
STRAND 559 561
HELIX 563 570
TURN 571 572
STRAND 573 575
STRAND 577 580
TURN 583 584
HELIX 585 591
STRAND 599 599
HELIX 600 603
TURN 604 605
Comments:
-!- FUNCTION: Exhibits several catalytic activities, including ATP-
dependent exonuclease, ATP-stimulated endonuclease, ATP-dependent
unwinding and DNA-dependent ATPase activities. Strand cleavage
occurs 5' to 3' during the unwinding of duplex DNA at CHI
sequences, which locally stimulate recombination.
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage (in the presence of
ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-
phosphooligonucleotides.
-!- SUBUNIT: Consist of three subunits; recB, recC and recD.
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Sequence length: 608
MKLQKQLLEA VEHKQLRPLD VQFALTVAGD EHPAVTLAAA LLSHDAGEGH VCLPLSRLEN
NEASHPLLAT CVSEIGELQN WEECLLASQA VSRGDEPTPM ILCGDRLYLN RMWCNERTVA
RFFNEVNHAI EVDEALLAQT LDKLFPVSDE INWQKVAAAV ALTRRISVIS GGPGTGKTTT
VAKLLAALIQ MADGERCRIR LAAPTGKAAA RLTESLGKAL RQLPLTDEQK KRIPEDASTL
HRLLGAQPGS QRLRHHAGNP LHLDVLVVDE ASMIDLPMMS RLIDALPDHA RVIFLGDRDQ
LASVEAGAVL GDICAYANAG FTAERARQLS RLTGTHVPAG TGTEAASLRD SLCLLQKSYR
FGSDSGIGQL AAAINRGDKT AVKTVFQQDF TDIEKRLLQS GEDYIAMLEE ALAGYGRYLD
LLQARAEPDL IIQAFNEYQL LCALREGPFG VAGLNERIEQ FMQQKRKIHR HPHSRWYEGR
PVMIARNDSA LGLFNGDIGI ALDRGQGTRV WFAMPDGNIK SVQPSRLPEH ETTWAMTVHK
SQGSEFDHAA LILPSQRTPV VTRELVYTAV TRARRRLSLY ADERILSAAI ATRTERRSGL
AALFSSRE