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Description:
Exodeoxyribonuclease V beta chain (EC 3.1.11.5) (ExodeoxyribonucleaseV 135 kDa polypeptide).
Molecular weight: 1339
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
01-AUG-1988, sequence version 1.
07-MAR-2006, entry version 54.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EX5B_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X04581 | CAA28250.1 | - |
| EMBL | AF179304 | AAD56369.1 | - |
| EMBL | U29581 | AAB40467.1 | - |
| EMBL | U00096 | AAC75859.1 | - |
| EMBL | X06227 | CAA29577.1 | - |
| EMBL | X04582 | CAA28252.1 | - |
| PIR | A25532 | NCECX5. | |
| PDB | 1W36 | X-ray | B/E=1-1180. |
| GenomeReviews | U00096_GR | b2820.1 | |
| EchoBASE | EB0817 | -.1 | |
| EcoGene | EG10824 | recB. | |
| BioCyc | EcoCyc:EG10824-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR004586 | RecB. | |
| InterPro | IPR000212 | UvrD-helicase. | |
| PANTHER | PTHR11070 | UvrD-helicase.1 | 2. |
| Pfam | PF00580 | UvrD-helicase | 1. |
| TIGRFAMs | TIGR00609 | recB | 1. |
Keywords:
3D-structure; ATP-binding; Complete proteome; DNA damage; DNA repair; Endonuclease; Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=87066729; PubMed=3537960;
RA Finch P.W., Storey A., Chapman K.E., Brown K., Hickson I.D.,
RA Emmerson P.T.;
RT "Complete nucleotide sequence of the Escherichia coli recB gene.";
RL Nucleic Acids Res. 14:8573-8582(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=V1000;
RX MEDLINE=20229837; PubMed=10766864; DOI=10.1074/jbc.275.16.12261;
RA Arnold D.A., Kowalczykowski S.C.;
RT "Facilitated loading of RecA protein is essential to recombination by
RT RecBCD enzyme.";
RL J. Biol. Chem. 275:12261-12265(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RX MEDLINE=87040734; PubMed=3534791;
RA Finch P.W., Wilson R.E., Brown K., Hickson I.D., Emmerson P.T.;
RT "Complete nucleotide sequence of the Escherichia coli ptr gene
RT encoding protease III.";
RL Nucleic Acids Res. 14:7695-7703(1986).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1093-1180.
RX MEDLINE=87066730; PubMed=3537961;
RA Finch P.W., Storey A., Brown K., Hickson I.D., Emmerson P.T.;
RT "Complete nucleotide sequence of recD, the structural gene for the
RT alpha subunit of Exonuclease V of Escherichia coli.";
RL Nucleic Acids Res. 14:8583-8594(1986).
Feature:
CHAIN 1 1180 Exodeoxyribonuclease V beta chain.
/FTId=PRO_0000102046.
NP_BIND 23 30 ATP.
STRAND 3 4
STRAND 6 7
HELIX 10 12
STRAND 16 17
STRAND 19 22
TURN 25 26
STRAND 27 27
HELIX 29 41
TURN 42 43
STRAND 45 49
STRAND 51 52
HELIX 56 58
STRAND 59 64
HELIX 66 88
STRAND 89 90
STRAND 94 94
HELIX 95 103
STRAND 105 105
HELIX 107 123
STRAND 125 128
HELIX 129 139
TURN 140 140
HELIX 141 144
TURN 145 145
STRAND 148 149
STRAND 151 152
HELIX 157 172
TURN 173 174
HELIX 177 186
STRAND 188 189
HELIX 190 197
TURN 198 201
STRAND 202 204
STRAND 207 210
TURN 214 215
HELIX 218 234
TURN 235 236
STRAND 237 237
STRAND 240 241
STRAND 249 251
STRAND 253 253
TURN 254 254
HELIX 255 263
TURN 264 264
STRAND 267 268
STRAND 272 272
TURN 279 280
STRAND 281 282
HELIX 283 287
STRAND 309 309
TURN 311 311
HELIX 312 316
TURN 317 318
STRAND 319 319
STRAND 323 323
HELIX 324 346
TURN 347 347
STRAND 348 349
HELIX 351 363
STRAND 364 364
TURN 365 366
HELIX 367 377
STRAND 379 383
STRAND 385 385
HELIX 386 388
HELIX 391 401
TURN 402 403
TURN 405 406
STRAND 408 413
HELIX 415 417
TURN 421 424
HELIX 427 436
STRAND 440 442
STRAND 445 446
TURN 447 448
STRAND 449 449
HELIX 451 462
STRAND 463 466
TURN 467 468
STRAND 469 470
TURN 471 472
STRAND 473 473
STRAND 479 480
HELIX 482 484
TURN 485 486
STRAND 487 491
TURN 492 493
STRAND 494 496
STRAND 498 503
STRAND 506 507
TURN 511 512
HELIX 513 534
TURN 535 536
STRAND 538 542
TURN 543 544
STRAND 545 548
HELIX 551 553
STRAND 554 560
HELIX 561 572
TURN 573 575
STRAND 578 580
TURN 581 582
STRAND 586 586
HELIX 587 589
STRAND 590 591
HELIX 592 603
TURN 604 604
TURN 606 607
STRAND 608 608
HELIX 609 617
STRAND 618 618
HELIX 619 621
TURN 622 622
HELIX 625 633
HELIX 635 655
HELIX 657 667
TURN 668 669
HELIX 670 676
STRAND 677 679
HELIX 680 698
TURN 699 700
STRAND 702 702
HELIX 704 716
STRAND 717 717
STRAND 722 722
TURN 723 724
STRAND 725 725
TURN 730 731
HELIX 732 734
STRAND 735 740
TURN 741 744
STRAND 745 746
STRAND 749 754
TURN 755 758
STRAND 765 765
STRAND 767 769
TURN 771 773
STRAND 776 781
HELIX 784 806
TURN 807 808
STRAND 809 817
STRAND 823 823
STRAND 825 825
STRAND 828 829
HELIX 832 835
STRAND 836 836
HELIX 837 842
TURN 843 843
STRAND 845 846
HELIX 850 859
STRAND 860 860
STRAND 862 862
TURN 863 864
STRAND 865 869
STRAND 901 906
STRAND 908 910
STRAND 912 913
TURN 915 916
HELIX 917 920
TURN 922 923
STRAND 924 924
TURN 926 927
STRAND 934 934
STRAND 938 939
HELIX 942 944
STRAND 945 945
STRAND 948 948
HELIX 949 958
TURN 959 960
STRAND 961 961
STRAND 964 966
HELIX 970 979
TURN 980 981
HELIX 984 998
STRAND 999 1000
STRAND 1002 1006
HELIX 1009 1011
HELIX 1014 1016
STRAND 1017 1027
STRAND 1029 1029
HELIX 1033 1043
STRAND 1045 1046
TURN 1047 1048
STRAND 1049 1049
STRAND 1055 1055
STRAND 1059 1074
STRAND 1076 1076
STRAND 1079 1082
STRAND 1088 1089
HELIX 1090 1092
STRAND 1093 1094
HELIX 1095 1104
TURN 1105 1107
HELIX 1108 1125
STRAND 1126 1128
HELIX 1131 1134
STRAND 1139 1145
TURN 1148 1149
TURN 1151 1152
STRAND 1153 1154
STRAND 1157 1157
STRAND 1162 1163
HELIX 1164 1173
Comments:
-!- FUNCTION: Required for efficient DNA repair; it catalyzes the
unwinding of double-stranded DNA and the cleavage of single-
stranded DNA and it stimulates local genetic recombination. All of
these activities require concomitant hydrolysis of ATP.
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage (in the presence of
ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-
phosphooligonucleotides.
-!- SUBUNIT: Consist of three subunits; recB, recC and recD.
-!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
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Sequence length: 1180
MSDVAETLDP LRLPLQGERL IEASAGTGKT FTIAALYLRL LLGLGGSAAF PRPLTVEELL
VVTFTEAATA ELRGRIRSNI HELRIACLRE TTDNPLYERL LEEIDDKAQA AQWLLLAERQ
MDEAAVFTIH GFCQRMLNLN AFESGMLFEQ QLIEDESLLR YQACADFWRR HCYPLPREIA
QVVFETWKGP QALLRDINRY LQGEAPVIKA PPPDDETLAS RHAQIVARID TVKQQWRDAV
GELDALIESS GIDRRKFNRS NQAKWIDKIS AWAEEETNSY QLPESLEKFS QRFLEDRTKA
GGETPRHPLF EAIDQLLAEP LSIRDLVITR ALAEIRETVA REKRRRGELG FDDMLSRLDS
ALRSESGEVL AAAIRTRFPV AMIDEFQDTD PQQYRIFRRI WHHQPETALL LIGDPKQAIY
AFRGADIFTY MKARSEVHAH YTLDTNWRSA PGMVNSVNKL FSQTDDAFMF REIPFIPVKS
AGKNQALRFV FKGETQPAMK MWLMEGESCG VGDYQSTMAQ VCAAQIRDWL QAGQRGEALL
MNGDDARPVR ASDISVLVRS RQEAAQVRDA LTLLEIPSVY LSNRDSVFET LEAQEMLWLL
QAVMTPEREN TLRSALATSM MGLNALDIET LNNDEHAWDV VVEEFDGYRQ IWRKRGVMPM
LRALMSARNI AENLLATAGG ERRLTDILHI SELLQEAGTQ LESEHALVRW LSQHILEPDS
NASSQQMRLE SDKHLVQIVT IHKSKGLEYP LVWLPFITNF RVQEQAFYHD RHSFEAVLDL
NAAPESVDLA EAERLAEDLR LLYVALTRSV WHCSLGVAPL VRRRGDKKGD TDVHQSALGR
LLQKGEPQDA AGLRTCIEAL CDDDIAWQTA QTGDNQPWQV NDVSTAELNA KTLQRLPGDN
WRVTSYSGLQ QRGHGIAQDL MPRLDVDAAG VASVVEEPTL TPHQFPRGAS PGTFLHSLFE
DLDFTQPVDP NWVREKLELG GFESQWEPVL TEWITAVLQA PLNETGVSLS QLSARNKQVE
MEFYLPISEP LIASQLDTLI RQFDPLSAGC PPLEFMQVRG MLKGFIDLVF RHEGRYYLLD
YKSNWLGEDS SAYTQQAMAA AMQAHRYDLQ YQLYTLALHR YLRHRIADYD YEHHFGGVIY
LFLRGVDKEH PQQGIYTTRP NAGLIALMDE MFAGMTLEEA