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Description:
Helicase IV (EC 3.6.1.-) (75 kDa helicase).
Molecular weight: 77976
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-APR-1990, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 54.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein HELD_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | J04726 | AAA23952.1 | - |
| EMBL | U00096 | AAC74048.1 | - |
| EMBL | D90733 | BAA35720.1 | - |
| EMBL | D90734 | BAA35727.1 | - |
| PIR | A64837 | HJECD4. | |
| HSSP | P09980 | 1UAA | |
| GenomeReviews | U00096_GR | b0962.1 | |
| EchoBASE | EB0421 | -.1 | |
| EcoGene | EG10426 | helD. | |
| BioCyc | EcoCyc:EG10426-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR000212 | UvrD-helicase. | |
| PANTHER | PTHR11070 | UvrD-helicase.1 | 1. |
| Pfam | PF00580 | UvrD-helicase | 2. |
Keywords:
ATP-binding; Complete proteome; Direct protein sequencing; DNA-binding; Helicase; Hydrolase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-27.
RX MEDLINE=89255269; PubMed=2542273;
RA Wood E.R., Matson S.W.;
RT "The molecular cloning of the gene encoding the Escherichia coli 75-
RT kDa helicase and the determination of its nucleotide sequence and
RT gentic map position.";
RL J. Biol. Chem. 264:8297-8303(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110;
RX MEDLINE=97061202; PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
RA Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
RA Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
RA Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
RA Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
RA Yano M., Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
Feature:
CHAIN 1 684 Helicase IV.
/FTId=PRO_0000102049.
NP_BIND 216 223 ATP (Probable).
CONFLICT 70 70 H -> P (in Ref. 1).
CONFLICT 137 140 SALP -> LRVA (in Ref. 1).
CONFLICT 365 366 MH -> ID (in Ref. 1).
Comments:
-!- FUNCTION: Helicase IV catalyzes the unwinding of duplex DNA in the
3' to 5' direction with respect to the bound single strand in a
reaction that is dependent upon the hydrolysis of ATP.
-!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
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Sequence length: 684
MELKATTLGK RLAQHPYDRA VILNAGIKVS GDRHEYLIPF NQLLAIHCKR GLVWGELEFV
LPDEKVVRLH GTEWGETQRF YHHLDAHWRR WSGEMSEIAS GVLRQQLDLI ATRTGENKWL
TREQTSGVQQ QIRQALSALP LPVNRLEEFD NCREAWRKCQ AWLKDIESAR LQHNQAYTEA
MLTEYADFFR QVESSPLNPA QARAVVNGEH SLLVLAGAGS GKTSVLVARA GWLLARGEAS
PEQILLLAFG RKAAEEMDER IRERLHTEDI TARTFHALAL HIIQQGSKKV PIVSKLENDT
AARHELFIAE WRKQCSEKKA QAKGWRQWLT EEMQWSVPEG NFWDDEKLQR RLASRLDRWV
SLMRMHGGAQ AEMIASAPEE IRDLFSKRIK LMAPLLKAWK GALKAENAVD FSGLIHQAIV
ILEKGRFISP WKHILVDEFQ DISPQRAALL AALRKQNSQT TLFAVGDDWQ AIYRFSGAQM
SLTTAFHENF GEGERCDLDT TYRFNSRIGE VANRFIQQNP GQLKKPLNSL TNGDKKAVTL
LDESQLDALL DKLSGYAKPE ERILILARYH HMRPASLEKA ATRWPKLQID FMTIHASKGQ
QADYVIIVGL QEGSDGFPAA ARESIMEEAL LPPVEDFPDA EERRLMYVAL TRARHRVWAL
FNKENPSPFV EILKNLDVPV ARKP