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Description:
G/U mismatch-specific DNA glycosylase (EC 3.2.2.-) (Mismatch-specificuracil DNA-glycosylase) (UDG).
Molecular weight: 18673
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
19-JUL-2005, sequence version 1.
07-MAR-2006, entry version 9.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MUG_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U28379 | AAA89148.1 | - |
| EMBL | U00096 | AAC76104.1 | - |
| EMBL | J01687 | NOT_ANNOTATED_CDS | |
| PIR | B65095 | B65095. | |
| PDB | 1MTL | X-ray | A/B=1-168. |
| PDB | 1MUG | X-ray | A=1-168. |
| PDB | 1MWI | X-ray | A=1-168. |
| PDB | 1MWJ | X-ray | A=1-168. |
| GenomeReviews | U00096_GR | b3068.1 | |
| EchoBASE | EB2576 | -.1 | |
| EcoGene | EG12717 | mug. | |
| BioCyc | EcoCyc:EG12717-MONOMER | -.1 | |
| LinkHub | P0A9H1 | -.1 |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 118-168.
RC STRAIN=K12;
RX MEDLINE=82014879; PubMed=6269063;
RA Burton Z.F., Burgess R.R., Lin J., Moore D., Holder S., Gross C.A.;
RT "The nucleotide sequence of the cloned rpoD gene for the RNA
RT polymerase sigma subunit from E coli K12.";
RL Nucleic Acids Res. 9:2889-2903(1981).
RN [3]
RP IDENTIFICATION.
RX MEDLINE=96032851; PubMed=7567469;
RA Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C.,
RA Danchin A.;
RT "Detection of new genes in a bacterial genome using Markov models for
RT three gene classes.";
RL Nucleic Acids Res. 23:3554-3562(1995).
RN [4]
RP FUNCTION.
RX MEDLINE=97032737; PubMed=8878487; DOI=10.1038/383735a0;
RA Gallinari P., Jiricny J.;
RT "A new class of uracil-DNA glycosylases related to human thymine-DNA
RT glycosylase.";
RL Nature 383:735-738(1996).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
RX MEDLINE=98149349; PubMed=9489705; DOI=10.1016/S0092-8674(00)80904-6;
RA Barrett T.E., Savva R., Panayotou G., Brown T., Barlow T., Jiricny J.,
RA Pearl L.H.;
RT "Crystal structure of a G:T/U mismatch-specific DNA glycosylase:
RT mismatch recognition by complementary-strand interactions.";
RL Cell 92:117-129(1998).
Feature:
CHAIN 1 168 G/U mismatch-specific DNA glycosylase.
/FTId=PRO_0000185774.
STRAND 5 5
TURN 8 9
STRAND 12 16
HELIX 21 26
TURN 27 27
TURN 29 30
TURN 33 34
HELIX 37 43
TURN 44 45
HELIX 53 62
TURN 63 63
STRAND 64 68
HELIX 77 79
HELIX 82 99
STRAND 103 107
HELIX 109 116
TURN 117 117
STRAND 124 130
TURN 131 132
STRAND 133 138
TURN 143 144
HELIX 149 162
Comments:
-!- FUNCTION: Removes uracil from G/U mispairs in ds-DNA. It is
capable of hydrolyzing the carbon-nitrogen bond between the sugar-
phosphate backbone of the DNA and a mispaired uracil.
-!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
-!- SIMILARITY: Belongs to the TDG/mug DNA glycosylase family.
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Sequence length: 168
MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL
DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG
AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR