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Description:
DNA mismatch repair protein mutH (Methyl-directed mismatch repairprotein).
Molecular weight: 25396
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) mismatch repair( GO:0006298 )
Important dates:
01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
01-FEB-1995, sequence version 2.
07-MAR-2006, entry version 61.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MUTH_ECOLI:
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24, AND
RP MUTANTS TS7 AND TS28.
RC STRAIN=K12;
RX MEDLINE=87174827; PubMed=3031619;
RA Grafstrom R.H., Hoess R.H.;
RT "Nucleotide sequence of the Escherichia coli mutH gene.";
RL Nucleic Acids Res. 15:3073-3084(1987).
RN [2]
RP SEQUENCE REVISION TO 82; 129-130 AND 135.
RC STRAIN=K12;
RA Feng G., Tsui H.T., Winkler M.E., Loh T.A., Marinus M.G.;
RL Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-156.
RC STRAIN=ECOR 1, ECOR 10, ECOR 13, ECOR 17, ECOR 20, ECOR 23, ECOR 24,
RC ECOR 26, ECOR 34, ECOR 35, ECOR 37, ECOR 4, ECOR 40, ECOR 41, ECOR 45,
RC ECOR 47, ECOR 49, ECOR 51, ECOR 52, ECOR 57, ECOR 58, ECOR 59,
RC ECOR 62, ECOR 64, ECOR 67, ECOR 68, ECOR 70, and
RC O2:HN / ECOR 50 / P97 / UPEC;
RA Denamur E.;
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
RX MEDLINE=98151376; PubMed=9482749; DOI=10.1093/emboj/17.5.1526;
RA Ban C., Yang W.;
RT "Structural basis for MutH activation in E.coli mismatch repair and
RT relationship of MutH to restriction endonucleases.";
RL EMBO J. 17:1526-1534(1998).
Feature:
INIT_MET 0 0
CHAIN 1 228 DNA mismatch repair protein mutH.
/FTId=PRO_0000198665.
VARIANT 57 57 W -> S (in strain: ECOR 67).
VARIANT 59 59 G -> C (in strain: ECOR 13, ECOR 20, ECOR
26 and ECOR 70).
VARIANT 63 63 G -> R (in strain: ECOR 50).
VARIANT 134 134 Q -> K (in strain: ECOR 51, ECOR 52, ECOR
57, ECOR 59, ECOR 62 and ECOR 64).
VARIANT 152 152 Q -> R (in strain: ECOR 67).
VARIANT 154 154 R -> C (in strain: ECOR 35).
MUTAGEN 27 27 T->L: In TS7; temperature-sensitive.
MUTAGEN 156 156 D->N: In TS28; temperature-sensitive.
STRAND 7 8
STRAND 11 12
HELIX 13 22
TURN 23 25
STRAND 27 27
HELIX 28 34
TURN 35 36
STRAND 41 41
TURN 43 45
TURN 47 48
HELIX 49 57
TURN 58 59
HELIX 68 71
STRAND 76 82
TURN 84 85
STRAND 86 86
STRAND 88 88
STRAND 90 90
STRAND 92 96
STRAND 99 101
TURN 103 104
TURN 107 109
HELIX 111 115
TURN 116 116
STRAND 117 125
TURN 128 129
STRAND 130 130
HELIX 132 134
STRAND 136 137
STRAND 141 143
HELIX 147 165
TURN 166 167
HELIX 169 171
TURN 174 175
STRAND 176 176
STRAND 178 183
STRAND 193 196
TURN 198 199
STRAND 200 200
STRAND 202 205
STRAND 209 212
HELIX 214 223
TURN 224 225
Comments:
-!- FUNCTION: Sequence-specific endonuclease that cleaves unmethylated
GATC sequences. It is involved in DNA mismatch repair.
-!- INTERACTION:
P23367:mutL; NbExp=2; IntAct=EBI-545170, EBI-554913;
-!- SUBCELLULAR LOCATION: Cytoplasm.
-!- SIMILARITY: Belongs to the mutH family.
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Sequence length: 228
SQPRPLLSPP ETEEQLLAQA QQLSGYTLGE LAALVGLVTP ENLKRDKGWI GVLLEIWLGA
SAGSKPEQDF AALGVELKTI PVDSLGRPLE TTFVCVAPLT GNSGVTWETS HVRHKLKRVL
WIPVEGERSI PLAQRRVGSP LLWSPNEEED RQLREDWEEL MDMIVLGQVE RITARHGEYL
QIRPKAANAK ALTEAIGARG ERILTLPRGF YLKKNFTSAL LARHFLIQ