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Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 19179
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
20-DEC-2005, sequence version 1.
07-MAR-2006, entry version 4.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | Y00495 | CAA68548.1 | - |
| EMBL | D90771 | BAA14928.1 | - |
| EMBL | D90772 | BAA14938.1 | - |
| EMBL | U00096 | AAC74417.1 | - |
| PIR | B64883 | XUECAD. | |
| HSSP | P16455 | 1QNT | |
| GenomeReviews | U00096_GR | b1335.1 | |
| EchoBASE | EB0662 | -.1 | |
| EcoGene | EG10668 | ogt. | |
| BioCyc | EcoCyc:EG10668-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX MEDLINE=88067749; PubMed=2825131;
RA Potter P.M., Wilkinson M.C., Fitton J., Carr F.J., Brennand J.,
RA Cooper D.P., Margison G.P.;
RT "Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-
RT DNA-alkyltransferase gene of E. coli.";
RL Nucleic Acids Res. 15:9177-9193(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251357; PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
RA Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
RA Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
RA Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
RA Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP MUTAGENESIS OF CYSTEINE RESIDUES.
RX MEDLINE=92392360; PubMed=1520330;
RA Harris L.C., Potter P.M., Margison G.P.;
RT "Site directed mutagenesis of two cysteine residues in the E. coli ogt
RT O6-alkylguanine DNA alkyltransferase protein.";
RL Biochem. Biophys. Res. Commun. 187:425-431(1992).
Feature:
CHAIN 1 171 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139365.
ACT_SITE 139 139 Alkyl group acceptor (Probable).
MUTAGEN 102 102 C->G: Little effect on the activity.
MUTAGEN 139 139 C->G: Eliminates the activity.
CONFLICT 37 37 R -> A (in Ref. 1).
CONFLICT 68 68 E -> D (in Ref. 1).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated. Can also
repair O-4-methylthymine.
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- INTERACTION:
P06958:aceE; NbExp=1; IntAct=EBI-555455, EBI-542683;
P06959:aceF; NbExp=1; IntAct=EBI-555455, EBI-542707;
P06977:gapA; NbExp=1; IntAct=EBI-555455, EBI-368904;
P0A6F5:groL; NbExp=1; IntAct=EBI-555455, EBI-543750;
P00391:lpdA; NbExp=1; IntAct=EBI-555455, EBI-542856;
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 171
MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG YERISATNPG
GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI PCGQVMHYGQ LAEQLGRPGA
ARAVGAANGS NPISIVVPCH RVIGRNGTMT GYAGGVQRKE WLLRHEGYLL L