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Description:
DNA replication and repair protein recF.
Molecular weight: 40383
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 1.
07-MAR-2006, entry version 10.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RECF_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | K02179 | AAA24511.1 | - |
| EMBL | X04341 | CAA27870.1 | - |
| EMBL | L10328 | AAA62051.1 | - |
| EMBL | U00096 | AAC76723.1 | - |
| PIR | A03547 | RQECF. | |
| HSSP | P58301 | 1F2T | |
| GenomeReviews | U00096_GR | b3700.1 | |
| EchoBASE | EB0821 | -.1 | |
| EcoGene | EG10828 | recF. | |
| GO | GO:0005515 | F:protein binding | IPI. |
| HAMAP | MF_00365 | - | 1. |
| InterPro | IPR001238 | RecF. | |
| InterPro | IPR002017 | Spectrin. | |
| Pfam | PF02463 | SMC_N | 1. |
| TIGRFAMs | TIGR00611 | recf | 1. |
| PROSITE | PS00617 | RECF_1 | 1. |
| PROSITE | PS00618 | RECF_2 | 1. |
Keywords:
ATP-binding; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA-binding; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=84272685; PubMed=6379647;
RA Blanar M.A., Sandler S.J., Armengod M.-E., Ream L.W., Clark A.J.;
RT "Molecular analysis of the recF gene of Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:4622-4626(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=84297235; PubMed=6089112;
RA Adachi T., Mizuuchi K., Menzel R., Gellert M.;
RT "DNA sequence and transcription of the region upstream of the E. coli
RT gyrB gene.";
RL Nucleic Acids Res. 12:6389-6395(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=93315143; PubMed=7686882;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli
RT genome: organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP PROTEIN SEQUENCE OF 1-12, AND DNA-BINDING.
RX MEDLINE=91035238; PubMed=2228960;
RA Griffin T.J. IV, Kolodner R.D.;
RT "Purification and preliminary characterization of the Escherichia coli
RT K-12 recF protein.";
RL J. Bacteriol. 172:6291-6299(1990).
RN [6]
RP MUTAGENESIS OF LYS-35.
RX MEDLINE=92178981; PubMed=1542576;
RA Sandler S.J., Chackerian B., Li J.T., Clark A.J.;
RT "Sequence and complementation analysis of recF genes from Escherichia
RT coli, Salmonella typhimurium, Pseudomonas putida and Bacillus
RT subtilis: evidence for an essential phosphate binding loop.";
RL Nucleic Acids Res. 20:839-845(1992).
Feature:
INIT_MET 0 0
CHAIN 1 356 DNA replication and repair protein recF.
/FTId=PRO_0000196414.
NP_BIND 29 36 ATP (Potential).
MUTAGEN 35 35 K->R: Weakly active.
Comments:
-!- FUNCTION: The recF protein is involved in DNA metabolism; it is
required for DNA replication and normal SOS inducibility. RecF
binds preferentially to single-stranded, linear DNA. It also seems
to bind ATP.
-!- INTERACTION:
P0A6F5:groL; NbExp=1; IntAct=EBI-556839, EBI-543750;
P0A8L1:serS; NbExp=1; IntAct=EBI-556839, EBI-548422;
Q47706:uidC; NbExp=1; IntAct=EBI-556839, EBI-556875;
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: Belongs to the recF family.
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Sequence length: 356
SLTRLLIRDF RNIETADLAL SPGFNFLVGA NGSGKTSVLE AIYTLGHGRA FRSLQIGRVI
RHEQEAFVLH GRLQGEERET AIGLTKDKQG DSKVRIDGTD GHKVAELAHL MPMQLITPEG
FTLLNGGPKY RRAFLDWGCF HNEPGFFTAW SNLKRLLKQR NAALRQVTRY EQLRPWDKEL
IPLAEQISTW RAEYSAGIAA DMADTCKQFL PEFSLTFSFQ RGWEKETEYA EVLERNFERD
RQLTYTAHGP HKADLRIRAD GAPVEDTLSR GQLKLLMCAL RLAQGEFLTR ESGRRCLYLI
DDFASELDDE RRGLLASRLK ATQSQVFVSA ISAEHVIDMS DENSKMFTVE KGKITD