|
|
|
|
|
|
Description:
ATP-dependent DNA helicase recG (EC 3.6.1.-).
Molecular weight: 76430
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
01-MAR-1992, sequence version 1.
07-MAR-2006, entry version 54.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RECG_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X59550 | CAA42123.1 | - |
| EMBL | M64367 | AAA24513.1 | - |
| EMBL | L10328 | AAA62005.1 | - |
| EMBL | U00096 | AAC76676.1 | - |
| PIR | JH0265 | JH0265. | |
| HSSP | Q9WY48 | 1GM5 | |
| GenomeReviews | U00096_GR | b3652.1 | |
| EchoBASE | EB0822 | -.1 | |
| EcoGene | EG10829 | recG. | |
| BioCyc | EcoCyc:EG10829-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR004365 | OB_tRNA_NA_bd. | |
| InterPro | IPR004609 | RecG. | |
| Pfam | PF00270 | DEAD | 1. |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF01336 | tRNA_anti | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00643 | recG | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=92041567; PubMed=1938888;
RA Lloyd R.G., Sharples G.J.;
RT "Molecular organization and nucleotide sequence of the recG locus of
RT Escherichia coli K-12.";
RL J. Bacteriol. 173:6837-6843(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=92184121; PubMed=1544582; DOI=10.1016/0378-1119(92)90449-Y;
RA Kalman M., Murphy H., Cashel M.;
RT "The nucleotide sequence of recG, the distal spo operon gene in
RT Escherichia coli K-12.";
RL Gene 110:95-99(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=93315143; PubMed=7686882;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli
RT genome: organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [4]
RP FUNCTION.
RX MEDLINE=95045415; PubMed=7957087;
RA Whitby M.C., Vincent S.D., Lloyd R.G.;
RT "Branch migration of Holliday junctions: identification of RecG
RT protein as a junction specific DNA helicase.";
RL EMBO J. 13:5220-5228(1994).
RN [5]
RP FUNCTION.
RX MEDLINE=95292979; PubMed=7774596;
RA Hong X., Cadwell G.W., Kogoma T.;
RT "Escherichia coli RecG and RecA proteins in R-loop formation.";
RL EMBO J. 14:2385-2392(1995).
Feature:
CHAIN 1 693 ATP-dependent DNA helicase recG.
/FTId=PRO_0000102140.
NP_BIND 296 303 ATP (Potential).
MOTIF 397 400 DEQH box.
Comments:
-!- FUNCTION: Critical role in recombination and DNA repair. Help
process Holliday junction intermediates to mature products by
catalyzing branch migration. Has a DNA unwinding activity
characteristic of a DNA helicase with a 3' to 5' polarity. recG
unwind branched duplex DNA (Y-DNA). Has a role in constitutive
stable DNA replication (csdR) and R-loop formation.
-!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 693
MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL YPIGELLPGV
YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS AAMKNSLAAG RRVLAYGEAK
RGKYGAEMIH PEYRVQGDLS TPELQETLTP VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE
ELLPPELSQG MMTLPEALRT LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR
AGAQRFHAQP LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS
GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW LAGKQKGKAR
LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH RFGVHQRLAL WEKGQQQGFH
PHQLIMTATP IPRTLAMTAY ADLDTSVIDE LPPGRTPVTT VAIPDTRRTD IIDRVHHACI
TEGRQAYWVC TLIEESELLE AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK
QGELHLLVAT TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT
PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD LLRDQAMIPE
VQRLARHIHE RYPQQAKALI ERWMPETERY SNA