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Description:
Single-stranded-DNA-specific exonuclease recJ (EC 3.1.-.-).
Molecular weight: 63389
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 43.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RECJ_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M54884 | AAA62789.1 | - |
| EMBL | U28375 | AAA83073.1 | - |
| EMBL | U00096 | AAC75930.1 | - |
| PIR | D65073 | D65073. | |
| HSSP | Q93R48 | 1IR6 | |
| GenomeReviews | U00096_GR | b2892.1 | |
| EchoBASE | EB0823 | -.1 | |
| EcoGene | EG10830 | recJ. | |
| BioCyc | EcoCyc:EG10830-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR003156 | Pesterase_DHHA1. | |
| InterPro | IPR001667 | Pesterase_RecJ. | |
| InterPro | IPR004610 | RecJ. | |
| Pfam | PF01368 | DHH | 1. |
| Pfam | PF02272 | DHHA1 | 1. |
| TIGRFAMs | TIGR00644 | recJ | 1. |
Keywords:
Complete proteome; Direct protein sequencing; Exonuclease; Hydrolase; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=91100304; PubMed=1987126;
RA Lovett S.T., Kolodner R.D.;
RT "Nucleotide sequence of the Escherichia coli recJ chromosomal region
RT and construction of recJ-overexpression plasmids.";
RL J. Bacteriol. 173:353-364(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [3]
RP FUNCTION, AND PROTEIN SEQUENCE OF 1-18.
RX MEDLINE=89202382; PubMed=2649886;
RA Lovett S.T., Kolodner R.D.;
RT "Identification and purification of a single-stranded-DNA-specific
RT exonuclease encoded by the recJ gene of Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:2627-2631(1989).
Feature:
CHAIN 1 577 Single-stranded-DNA-specific exonuclease
recJ.
/FTId=PRO_0000097228.
CONFLICT 6 6 Q -> H (in Ref. 1).
CONFLICT 20 20 A -> AA (in Ref. 1).
Comments:
-!- FUNCTION: Single-stranded-DNA-specific exonuclease. Required for
many types of recombinational events, although the stringency of
the requirement for recJ appears to vary with the type of
recombinational event monitored and the other recombination gene
products which are available.
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Sequence length: 577
MKQQIQLRRR EVDETADLPA ELPPLLRRLY ASRGVRSAQE LERSVKGMLP WQQLSGVEKA
VEILYNAFRE GTRIIVVGDF DADGATSTAL SVLAMRSLGC SNIDYLVPNR FEDGYGLSPE
VVDQAHARGA QLIVTVDNGI SSHAGVEHAR SLGIPVIVTD HHLPGDTLPA AEAIINPNLR
DCNFPSKSLA GVGVAFYLML ALRTFLRDQG WFDERNIAIP NLAELLDLVA LGTVADVVPL
DANNRILTWQ GMSRIRAGKC RPGIKALLEV ANRDAQKLAA SDLGFALGPR LNAAGRLDDM
SVGVALLLCD NIGEARVLAN ELDALNQTRK EIEQGMQIEA LTLCEKLERS RDTLPGGLAM
YHPEWHQGVV GILASRIKER FHRPVIAFAP AGDGTLKGSG RSIQGLHMRD ALERLDTLYP
GMMLKFGGHA MAAGLSLEED KFKLFQQRFG ELVTEWLDPS LLQGEVVSDG PLSPAEMTME
VAQLLRDAGP WGQMFPEPLF DGHFRLLQQR LVGERHLKVM VEPVGGGPLL DGIAFNVDTA
LWPDNGVREV QLAYKLDINE FRGNRSLQII IDNIWPI