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Description:
ATP-dependent DNA helicase recQ (EC 3.6.1.-).
Molecular weight: 68232
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-APR-1990, integrated into UniProtKB/Swiss-Prot.
11-OCT-2004, sequence version 4.
07-MAR-2006, entry version 66.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RECQ_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M30198 | AAA24517.1 | ALT_INIT |
| EMBL | M87049 | AAA67618.1 | ALT_INIT |
| EMBL | U00096 | AAT48221.1 | - |
| PDB | 1OYW | X-ray | A=1-522. |
| PDB | 1OYY | X-ray | A=1-522. |
| PDB | 1WUD | X-ray | A/B/D=523-608. |
| GenomeReviews | U00096_GR | b3822.1 | |
| EchoBASE | EB0826 | -.1 | |
| EcoGene | EG10833 | recQ. | |
| BioCyc | EcoCyc:EG10833-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR006293 | ATP_RecQ. | |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR002464 | DEAH_box. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR002121 | HRDC. | |
| InterPro | IPR004589 | RecQ. | |
| InterPro | IPR002857 | Znf_CXXC. | |
| PANTHER | PTHR13710 | RecQ.1 | 1. |
| Pfam | PF00270 | DEAD | 1. |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF00570 | HRDC | 1. |
| Pfam | PF02008 | zf-CXXC | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| SMART | SM00341 | HRDC | 1. |
| TIGRFAMs | TIGR01389 | recQ | 1. |
| TIGRFAMs | TIGR00614 | recQ_fam | 1. |
| PROSITE | PS00690 | DEAH_ATP_HELICASE | FALSE_NEG. |
| PROSITE | PS50967 | HRDC | 1. |
Keywords:
3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=87115164; PubMed=3027506;
RA Irino N., Nakayama K., Nakayama H.;
RT "The recQ gene of Escherichia coli K12: primary structure and evidence
RT for SOS regulation.";
RL Mol. Gen. Genet. 205:298-304(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=92358234; PubMed=1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region
RT from 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION
RP TO 254.
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP SEQUENCE REVISION TO 502-503.
RA Arnaud M., Berlyn M.K.B., Blattner F.R., Galperin M.Y., Glasner J.D.,
RA Horiuchi T., Kosuge T., Mori H., Perna N.T., Plunkett G. III,
RA Riley M., Rudd K.E., Serres M.H., Thomas G.H., Wanner B.L.;
RT "Workshop on annotation of Escherichia coli K-12.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND PROTEIN SEQUENCE OF 1-5.
RX MEDLINE=90319113; PubMed=2164680;
RA Umezu K., Nakayama K., Nakayama H.;
RT "Escherichia coli RecQ protein is a DNA helicase.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:5363-5367(1990).
Feature:
INIT_MET 0 0
CHAIN 1 608 ATP-dependent DNA helicase recQ.
/FTId=PRO_0000205033.
DOMAIN 528 608 HRDC.
NP_BIND 46 53 ATP (Potential).
MOTIF 145 148 DEAH box.
CONFLICT 254 254 R -> A (in Ref. 1 and 2).
CONFLICT 502 503 RG -> A (in Ref. 1 and 2).
CONFLICT 599 599 R -> P (in Ref. 1).
STRAND 6 7
HELIX 8 18
TURN 19 20
STRAND 23 24
TURN 27 28
HELIX 29 37
TURN 38 39
STRAND 42 45
STRAND 47 47
HELIX 49 62
STRAND 63 70
STRAND 72 72
HELIX 74 86
TURN 87 88
STRAND 91 94
TURN 96 97
STRAND 98 98
HELIX 100 111
TURN 112 113
STRAND 116 120
HELIX 122 125
TURN 126 126
STRAND 127 127
TURN 128 129
HELIX 130 134
TURN 135 136
STRAND 137 137
STRAND 139 146
HELIX 147 150
TURN 152 153
STRAND 154 155
HELIX 159 170
TURN 172 173
STRAND 174 174
STRAND 176 181
HELIX 185 195
TURN 196 196
STRAND 199 199
STRAND 201 204
TURN 210 211
STRAND 212 218
STRAND 220 221
HELIX 222 232
TURN 233 235
STRAND 238 241
STRAND 243 244
HELIX 245 257
TURN 258 259
STRAND 262 265
TURN 267 268
STRAND 269 269
HELIX 271 282
TURN 283 284
STRAND 285 285
STRAND 287 291
TURN 293 294
STRAND 295 295
TURN 297 299
TURN 302 303
STRAND 306 311
STRAND 314 315
HELIX 316 323
TURN 324 325
STRAND 326 326
TURN 328 329
STRAND 330 331
STRAND 333 339
HELIX 341 352
TURN 353 353
STRAND 354 354
STRAND 357 357
HELIX 358 374
TURN 375 375
STRAND 377 378
HELIX 380 387
TURN 388 389
STRAND 394 394
STRAND 397 397
STRAND 399 399
HELIX 400 403
STRAND 404 404
STRAND 408 410
HELIX 412 424
TURN 425 427
HELIX 431 439
TURN 440 440
HELIX 444 448
TURN 449 450
HELIX 451 453
STRAND 454 454
TURN 455 462
STRAND 463 463
HELIX 465 477
TURN 478 479
STRAND 480 483
HELIX 485 487
TURN 488 489
STRAND 491 493
HELIX 495 501
TURN 502 503
STRAND 504 505
STRAND 508 510
HELIX 532 549
TURN 550 550
STRAND 551 551
HELIX 553 556
HELIX 559 568
STRAND 572 572
HELIX 573 577
TURN 578 578
STRAND 579 579
TURN 580 581
HELIX 584 602
TURN 603 604
Comments:
-!- FUNCTION: Involved in the recF recombination pathway; its gene
expression is under the regulation of the SOS system. It is a DNA
helicase.
-!- SIMILARITY: Belongs to the helicase family. RecQ subfamily.
-!- SIMILARITY: Contains 1 HRDC domain.
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Sequence length: 608
AQAEVLNLES GAKQVLQETF GYQQFRPGQE EIIDTVLSGR DCLVVMPTGG GKSLCYQIPA
LLLNGLTVVV SPLISLMKDQ VDQLQANGVA AACLNSTQTR EQQLEVMTGC RTGQIRLLYI
APERLMLDNF LEHLAHWNPV LLAVDEAHCI SQWGHDFRPE YAALGQLRQR FPTLPFMALT
ATADDTTRQD IVRLLGLNDP LIQISSFDRP NIRYMLMEKF KPLDQLMRYV QEQRGKSGII
YCNSRAKVED TAARLQSKGI SAAAYHAGLE NNVRADVQEK FQRDDLQIVV ATVAFGMGIN
KPNVRFVVHF DIPRNIESYY QETGRAGRDG LPAEAMLFYD PADMAWLRRC LEEKPQGQLQ
DIERHKLNAM GAFAEAQTCR RLVLLNYFGE GRQEPCGNCD ICLDPPKQYD GSTDAQIALS
TIGRVNQRFG MGYVVEVIRG ANNQRIRDYG HDKLKVYGMG RDKSHEHWVS VIRQLIHLGL
VTQNIAQHSA LQLTEAARPV LRGESSLQLA VPRIVALKPK AMQKSFGGNY DRKLFAKLRK
LRKSIADESN VPPYVVFNDA TLIEMAEQMP ITASEMLSVN GVGMRKLERF GKPFMALIRA
HVDGDDEE