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Description:
Regulatory protein recX (Protein oraA).
Molecular weight: 19424
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) regulation of DNA repair( GO:0006282 )
Important dates:
01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 43.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RECX_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L25853 | AAA19106.1 | - |
| EMBL | U00096 | AAC75740.1 | - |
| EMBL | D90892 | BAA16560.1 | - |
| PIR | F65049 | F65049. | |
| GenomeReviews | U00096_GR | b2698.1 | |
| EchoBASE | EB2005 | -.1 | |
| EcoGene | EG12080 | recX. | |
| BioCyc | EcoCyc:EG12080-MONOMER | -.1 | |
| HAMAP | MF_01114 | - | 1. |
| InterPro | IPR003783 | RecX. | |
| Pfam | PF02631 | RecX | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / CS520;
RX PubMed=7512687; DOI=10.1016/0165-7992(94)90030-2;
RA Zaitsev E., Alexseyev A., Lanzov V., Satin L., Clark A.J.;
RT "Nucleotide sequence between recA and alaSp in E. coli K12 and the
RT sequence change in four recA mutations.";
RL Mutat. Res. 323:173-177(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97349980; PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K.,
RA Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N.,
RA Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H.,
RA Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S.,
RA Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C.,
RA Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-
RT K12 genome corresponding to 50.0-68.8 min on the linkage map and
RT analysis of its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [4]
RP FUNCTION.
RX MEDLINE=22421375; PubMed=12427742; DOI=10.1074/jbc.M210496200;
RA Stohl E.A., Brockman J.P., Burkle K.L., Morimatsu K.,
RA Kowalczykowski S.C., Seifert H.S.;
RT "Escherichia coli RecX inhibits RecA recombinase and coprotease
RT activities in vitro and in vivo.";
RL J. Biol. Chem. 278:2278-2285(2003).
RN [5]
RP SIMILARITY.
RX MEDLINE=94218258; PubMed=8165147;
RA de Mot R., Schoofs G., Vanderleyden J.;
RT "A putative regulatory gene downstream of recA is conserved in Gram-
RT negative and Gram-positive bacteria.";
RL Nucleic Acids Res. 22:1313-1314(1994).
Feature:
CHAIN 1 166 Regulatory protein recX.
/FTId=PRO_0000162429.
CONFLICT 19 19 V -> L (in Ref. 1).
CONFLICT 120 120 L -> P (in Ref. 1).
Comments:
-!- FUNCTION: Modulates recA activity through direct physical
interaction. Can inhibit both recA recombinase and coprotease
activities. May have a regulatory role during the SOS response.
Inhibits DNA strand exchange in vitro.
-!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
-!- INDUCTION: By DNA damage, via lexA, as part of the SOS response.
-!- SIMILARITY: Belongs to the recX family.
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Sequence length: 166
MTESTSRRPA YARLLDRAVR ILAVRDHSEQ ELRRKLAAPI MGKNGPEEID ATAEDYERVI
AWCHEHGYLD DSRFVARFIA SRSRKGYGPA RIRQELNQKG ISREATEKAM RECDIDWCAL
ARDQATRKYG EPLPTVFSEK VKIQRFLLYR GYLMEDIQEI WRNFAD