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Description:
Crossover junction endodeoxyribonuclease rusA (EC 3.1.22.-) (Hollidayjunction nuclease rusA) (Holliday juction resolvase).
Molecular weight: 13846
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
20-DEC-2005, sequence version 1.
07-MAR-2006, entry version 4.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUS_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X92587 | CAA63321.1 | - |
| EMBL | U82598 | AAB40746.1 | - |
| EMBL | U00096 | AAC73651.1 | - |
| PIR | S66590 | S66590. | |
| PDB | 1Q8R | X-ray | A/B=1-120. |
| GenomeReviews | U00096_GR | b0550.1 | |
| EchoBASE | EB4170 | -.1 | |
| EcoGene | EG20283 | rusA. | |
| BioCyc | EcoCyc:G6306-MONOMER | -.1 |
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase; Magnesium; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=96196428; PubMed=8648624; DOI=10.1006/jmbi.1996.0185;
RA Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.;
RT "Holliday junction resolvases encoded by homologous rusA genes in
RT Escherichia coli K-12 and phage 82.";
RL J. Mol. Biol. 257:561-573(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP PROTEIN SEQUENCE OF 1-9, AND CHARACTERIZATION.
RX MEDLINE=95112824; PubMed=7813450;
RA Sharples G.J., Chan S.N., Mahdi A.A., Whitby M.C., Lloyd R.G.;
RT "Processing of intermediates in recombination and DNA repair:
RT identification of a new endonuclease that specifically cleaves
RT Holliday junctions.";
RL EMBO J. 13:6133-6142(1994).
Feature:
CHAIN 1 120 Crossover junction endodeoxyribonuclease
rusA.
/FTId=PRO_0000192004.
STRAND 3 8
HELIX 14 17
STRAND 18 20
STRAND 22 27
HELIX 29 44
TURN 45 46
STRAND 51 51
STRAND 53 53
STRAND 55 62
STRAND 64 67
HELIX 71 73
STRAND 74 75
HELIX 76 84
TURN 85 86
STRAND 87 89
HELIX 91 93
STRAND 94 102
TURN 106 107
STRAND 109 116
Comments:
-!- FUNCTION: Endonuclease that resolves holliday junction
intermediates in genetic recombination. Cleaves X-junctions by
introducing symmetrical nicks in two strands of the same polarity.
Processes Holliday intermediates made by recA. Corrects the
defects in genetic recombination and DNA repair associated with
inactivation of ruvAB or ruvC.
-!- SIMILARITY: Belongs to the rusA family.
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Sequence length: 120
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG LAMPVKIRIE
CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE