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Description:
Holliday junction ATP-dependent DNA helicase ruvA (EC 3.6.1.-).
Molecular weight: 22086
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 1.
07-MAR-2006, entry version 13.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVA_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X07091 | CAA30119.1 | - |
| EMBL | M21298 | AAA24612.1 | - |
| EMBL | D90829 | BAA15672.1 | - |
| EMBL | U00096 | AAC74931.1 | - |
| EMBL | D10165 | BAA01034.1 | - |
| PIR | E64948 | BVECRV. | |
| PDB | 1BDX | X-ray | A/B/C/D=1-203. |
| PDB | 1C7Y | X-ray | A=1-203. |
| PDB | 1CUK | X-ray | @=1-203. |
| PDB | 1D8L | X-ray | A/B=1-149. |
| PDB | 1HJP | X-ray | @=1-203. |
| GenomeReviews | U00096_GR | b1861.1 | |
| EchoBASE | EB0916 | -.1 | |
| EcoGene | EG10923 | ruvA. | |
| LinkHub | P0A809 | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| HAMAP | MF_00031 | - | 1. |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR012340 | OB_NA_bd_sub. | |
| InterPro | IPR000085 | RuvA. | |
| Pfam | PF00633 | HHH | 2. |
| Pfam | PF07499 | RuvA_C | 1. |
| Pfam | PF01330 | RuvA_N | 1. |
| ProDom | PD006268 | RuvA | 1. |
| SMART | SM00278 | HhH1 | 2. |
| TIGRFAMs | TIGR00084 | ruvA | 1. |
Keywords:
3D-structure; ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=88157713; PubMed=3279394;
RA Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.;
RT "Nucleotide sequencing of the ruv region of Escherichia coli K-12
RT reveals a LexA regulated operon encoding two genes.";
RL Nucleic Acids Res. 16:1541-1549(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=88314937; PubMed=2842314;
RA Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.;
RT "Structure and regulation of the Escherichia coli ruv operon involved
RT in DNA repair and recombination.";
RL J. Bacteriol. 170:4322-4329(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
RX MEDLINE=91358366; PubMed=1885548;
RA Takahagi M., Iwasaki H., Nakata A., Shinagawa H.;
RT "Molecular analysis of the Escherichia coli ruvC gene, which encodes a
RT Holliday junction-specific endonuclease.";
RL J. Bacteriol. 173:5747-5753(1991).
RN [6]
RP CHARACTERIZATION.
RX MEDLINE=93165688; PubMed=8433990;
RA Tsaneva I.R., Mueller B., West S.C.;
RT "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase
RT activity in vitro.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX MEDLINE=96436563; PubMed=8832889; DOI=10.1126/science.274.5286.415;
RA Rafferty J.B., Sedelnikova S.E., Hargreaves D., Artymiuk P.J.,
RA Baker P.J., Sharples G.J., Mahdi A.A., Lloyd R.G., Rice D.W.;
RT "Crystal structure of DNA recombination protein RuvA and a model for
RT its binding to the Holliday junction.";
RL Science 274:415-421(1996).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (6.0 ANGSTROMS).
RX MEDLINE=98290445; PubMed=9628481;
RA Hargreaves D., Rice D.W., Sedelnikova S.E., Artymiuk P.J., Lloyd R.G.,
RA Rafferty J.B.;
RT "Crystal structure of E.coli RuvA with bound DNA Holliday junction at
RT 6-A resolution.";
RL Nat. Struct. Biol. 5:441-446(1998).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX MEDLINE=98154314; PubMed=9493263; DOI=10.1016/S0969-2126(98)00003-3;
RA Nishino T., Ariyoshi M., Iwasaki H., Shinagawa H., Morikawa K.;
RT "Functional analyses of the domain structure in the Holliday junction
RT binding protein RuvA.";
RL Structure 6:11-21(1998).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-149.
RX MEDLINE=20237723; PubMed=10772859; DOI=10.1006/jmbi.2000.3675;
RA Nishino T., Iwasaki H., Kataoka M., Ariyoshi M., Fujita T.,
RA Shinagawa H., Morikawa K.;
RT "Modulation of RuvB function by the mobile domain III of the Holliday
RT junction recognition protein RuvA.";
RL J. Mol. Biol. 298:407-416(2000).
RN [11]
RP REVIEW.
RX MEDLINE=98105106; PubMed=9442895; DOI=10.1146/annurev.genet.31.1.213;
RA West S.C.;
RT "Processing of recombination intermediates by the RuvABC proteins.";
RL Annu. Rev. Genet. 31:213-244(1997).
Feature:
CHAIN 1 203 Holliday junction ATP-dependent DNA
helicase ruvA.
/FTId=PRO_0000094629.
CONFLICT 166 166 A -> R (in Ref. 1).
STRAND 4 13
TURN 14 15
STRAND 16 21
TURN 22 23
STRAND 24 29
HELIX 32 35
TURN 36 37
TURN 41 42
STRAND 44 54
TURN 55 56
STRAND 57 63
HELIX 66 77
HELIX 83 92
HELIX 95 103
TURN 104 105
HELIX 107 111
TURN 112 112
TURN 114 115
HELIX 118 131
HELIX 132 134
TURN 138 140
HELIX 158 170
TURN 171 171
HELIX 174 183
HELIX 191 200
TURN 201 203
Comments:
-!- FUNCTION: The ruvA-ruvB complex in the presence of ATP renatures
cruciform structure in supercoiled DNA with palindromic sequence,
indicating that it may promote strand exchange reactions in
homologous recombination. RuvAB is an helicase that mediates the
Holliday junction migration by localized denaturation and
reannealing. RuvA stimulates, in the presence of DNA, the weak
ATPase activity of ruvB. Binds both single- and double-stranded
DNA (dsDNA). Binds preferentially to supercoiled rather than to
relaxed dsDNA.
-!- SUBUNIT: Homotetramer; forms a complex with ruvB.
-!- INTERACTION:
P68739:nfi; NbExp=1; IntAct=EBI-555119, EBI-551698;
P21893:recJ; NbExp=1; IntAct=EBI-555119, EBI-556893;
-!- INDUCTION: Expression of the ruv region is induced by damage to
DNA and is regulated by lexA as part of the SOS response. RuvA and
ruvB are also involved in mutagenesis induced by UV and X
irradiation and by some chemicals.
-!- SIMILARITY: Belongs to the ruvA family.
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Sequence length: 203
MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH FVVREDAQLL
YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN AVEREEVGAL VKLPGIGKKT
AERLIVEMKD RFKGLHGDLF TPAADLVLTS PASPATDDAE QEAVAALVAL GYKPQEASRM
VSKIARPDAS SETLIREALR AAL