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Description:
Holliday junction ATP-dependent DNA helicase ruvB (EC 3.6.1.-).
Molecular weight: 37174
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
01-AUG-1988, sequence version 1.
07-MAR-2006, entry version 11.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVB_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X07091 | CAA30120.1 | - |
| EMBL | M21298 | AAA24613.1 | - |
| EMBL | D90828 | BAA15668.1 | - |
| EMBL | D90829 | BAA15671.1 | - |
| EMBL | U00096 | AAC74930.1 | - |
| PIR | B28533 | BVECVB. | |
| HSSP | Q56313 | 1J7K | |
| GenomeReviews | U00096_GR | b1860.1 | |
| EchoBASE | EB0917 | -.1 | |
| EcoGene | EG10924 | ruvB. | |
| GO | GO:0005515 | F:protein binding | IPI. |
| HAMAP | MF_00016 | - | 1. |
| InterPro | IPR003593 | AAA_ATPase. | |
| InterPro | IPR001984 | Peptidase_S16. | |
| InterPro | IPR004605 | RuvB. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF00004 | AAA | 1. |
| Pfam | PF05491 | RuvB_C | 1. |
| Pfam | PF05496 | RuvB_N | 1. |
| PRINTS | PR00830 | ENDOLAPTASE. | |
| SMART | SM00382 | AAA | 1. |
| TIGRFAMs | TIGR00635 | ruvB | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; Helicase; Hydrolase; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=88157713; PubMed=3279394;
RA Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.;
RT "Nucleotide sequencing of the ruv region of Escherichia coli K-12
RT reveals a LexA regulated operon encoding two genes.";
RL Nucleic Acids Res. 16:1541-1549(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=88314937; PubMed=2842314;
RA Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.;
RT "Structure and regulation of the Escherichia coli ruv operon involved
RT in DNA repair and recombination.";
RL J. Bacteriol. 170:4322-4329(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP CHARACTERIZATION.
RX MEDLINE=93165688; PubMed=8433990;
RA Tsaneva I.R., Mueller B., West S.C.;
RT "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase
RT activity in vitro.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993).
RN [6]
RP REVIEW.
RX MEDLINE=98105106; PubMed=9442895; DOI=10.1146/annurev.genet.31.1.213;
RA West S.C.;
RT "Processing of recombination intermediates by the RuvABC proteins.";
RL Annu. Rev. Genet. 31:213-244(1997).
Feature:
CHAIN 1 336 Holliday junction ATP-dependent DNA
helicase ruvB.
/FTId=PRO_0000165528.
NP_BIND 62 69 ATP (Potential).
Comments:
-!- FUNCTION: The ruvA-ruvB complex in the presence of ATP renatures
cruciform structure in supercoiled DNA with palindromic sequence,
indicating that it may promote strand exchange reactions in
homologous recombination. RuvAB is an helicase that mediates the
Holliday junction migration by localized denaturation and
reannealing.
-!- ENZYME REGULATION: RuvB possesses weak ATPase activity, which is
stimulated by the ruvA protein in the presence of DNA.
-!- SUBUNIT: Homododecamer composed of two hexameric rings; when bound
to DNA in the presence of ATP and magnesium. Forms a complex with
ruvA.
-!- INDUCTION: Expression of the ruv region is induced by damage to
DNA and is regulated by lexA as part of the SOS response. RuvA and
ruvB are also involved in mutagenesis induced by UV and X
irradiation and by some chemicals.
-!- SIMILARITY: Belongs to the ruvB family.
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Sequence length: 336
MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL RGDALDHLLI
FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM LTNLEPHDVL FIDEIHRLSP
VVEEVLYPAM EDYQLDIMIG EGPAARSIKI DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR
LEFYQVPDLQ YIVSRSARFM GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG
TISADIAAQA LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV
LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP