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Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 18616
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 1.
07-MAR-2006, entry version 7.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X59551 | CAA42128.1 | - |
| EMBL | D10165 | BAA01032.1 | - |
| EMBL | D90829 | BAA15674.1 | - |
| EMBL | U00096 | AAC74933.1 | - |
| PIR | D38113 | D38113. | |
| PDB | 1HJR | X-ray | A/B/C/D=1-158. |
| GenomeReviews | U00096_GR | b1863.1 | |
| EchoBASE | EB0918 | -.1 | |
| EcoGene | EG10925 | ruvC. | |
| LinkHub | P0A814 | -.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| TIGRFAMs | TIGR00228 | ruvC | 1. |
| PROSITE | PS01321 | RUVC | 1. |
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=92041688; PubMed=1657895;
RA Sharples G.J., Lloyd R.G.;
RT "Resolution of Holliday junctions in Escherichia coli: identification
RT of the ruvC gene product as a 19-kilodalton protein.";
RL J. Bacteriol. 173:7711-7715(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=91358366; PubMed=1885548;
RA Takahagi M., Iwasaki H., Nakata A., Shinagawa H.;
RT "Molecular analysis of the Escherichia coli ruvC gene, which encodes a
RT Holliday junction-specific endonuclease.";
RL J. Bacteriol. 173:5747-5753(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP PROTEIN SEQUENCE OF 1-41, AND FUNCTION.
RX MEDLINE=92097558; PubMed=1661673;
RA Iwasaki H., Takahagi M., Shiba T., Nakata A., Shinagawa H.;
RT "Escherichia coli RuvC protein is an endonuclease that resolves the
RT Holliday structure.";
RL EMBO J. 10:4381-4389(1991).
RN [6]
RP PROTEIN SEQUENCE OF 1-24, AND FUNCTION.
RX MEDLINE=92100188; PubMed=1758493; DOI=10.1038/354506a0;
RA Dunderdale H.J., Benson F.E., Parsons C.A., Sharples G.J., Lloyd R.G.,
RA West S.C.;
RT "Formation and resolution of recombination intermediates by E. coli
RT RecA and RuvC proteins.";
RL Nature 354:506-510(1991).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX MEDLINE=95007756; PubMed=7923356; DOI=10.1016/0092-8674(94)90280-1;
RA Ariyoshi M., Vassylyev D.G., Iwasaki H., Nakamura H., Shinagawa H.,
RA Morikawa K.;
RT "Atomic structure of the RuvC resolvase: a holliday junction-specific
RT endonuclease from E. coli.";
RL Cell 78:1063-1072(1994).
RN [8]
RP REVIEW.
RX MEDLINE=98105106; PubMed=9442895; DOI=10.1146/annurev.genet.31.1.213;
RA West S.C.;
RT "Processing of recombination intermediates by the RuvABC proteins.";
RL Annu. Rev. Genet. 31:213-244(1997).
Feature:
INIT_MET 0 0
CHAIN 1 172 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183096.
METAL 7 7 Magnesium.
METAL 66 66 Magnesium.
METAL 138 138 Magnesium.
METAL 141 141 Magnesium.
STRAND 2 7
STRAND 12 21
TURN 22 23
STRAND 24 34
HELIX 40 58
STRAND 62 67
TURN 74 76
HELIX 77 92
TURN 93 95
STRAND 98 102
HELIX 103 110
TURN 111 111
HELIX 118 128
TURN 129 130
TURN 139 139
HELIX 140 151
TURN 152 153
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group.
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit.
-!- SUBUNIT: Homodimer.
-!- SIMILARITY: Belongs to the ruvC family.
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Sequence length: 172
AIILGIDPGS RVTGYGVIRQ VGRQLSYLGS GCIRTKVDDL PSRLKLIYAG VTEIITQFQP
DYFAIEQVFM AKNADSALKL GQARGVAIVA AVNQELPVFE YAARQVKQTV VGIGSAEKSQ
VQHMVRTLLK LPANPQADAA DALAIAITHC HVSQNAMQMS ESRLNLARGR LR