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Description:
Single-stranded DNA-binding protein (SSB) (Helix-destabilizingprotein).
Molecular weight: 18844
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
20-DEC-2005, sequence version 1.
07-MAR-2006, entry version 5.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein SSB_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | J01704 | AAA24649.1 | - |
| EMBL | U00006 | AAC43153.1 | - |
| EMBL | U00096 | AAC77029.1 | - |
| PIR | B65214 | DDEC. | |
| PDB | 1EQQ | X-ray | A/B/C/D=1-177. |
| PDB | 1EYG | X-ray | A/B/C/D=1-115. |
| PDB | 1KAW | X-ray | A/B/C/D=1-135. |
| PDB | 1QVC | X-ray | A/B/C/D=1-145. |
| PDB | 1SRU | X-ray | A/B/C/D=1-112. |
| SWISS-2DPAGE | P0AGE0 | COLI. | |
| ECO2DBASE | F018.8 | 6TH EDITION. | |
| GenomeReviews | U00096_GR | b4059.1 | |
| EchoBASE | EB0969 | -.1 | |
| EcoGene | EG10976 | ssb. | |
| BioCyc | EcoCyc:EG10976-MONOMER | -.1 | |
| PROSITE | PS50935 | SSB | 1. |
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-52.
RX MEDLINE=82037821; PubMed=6270666;
RA Sancar A., Williams K.R., Chase J.W., Rupp W.D.;
RT "Sequences of the ssb gene and protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 78:4274-4278(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=83299995; PubMed=6351061;
RA Chase J.W., Merrill B.M., Williams K.R.;
RT "F sex factor encodes a single-stranded DNA binding protein (SSB) with
RT extensive sequence homology to Escherichia coli SSB.";
RL Proc. Natl. Acad. Sci. U.S.A. 80:5480-5484(1983).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=94089392; PubMed=8265357;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J.,
RA Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the
RT region from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP PROTEIN SEQUENCE OF 1-40.
RX MEDLINE=82186711; PubMed=7042342;
RA Beyreuther K., Berthold-Schmidt V., Geider K.;
RT "Biological activity and a partial amino-acid sequence of Escherichia
RT coli DNA-binding protein I isolated from overproducing cells.";
RL Eur. J. Biochem. 123:415-420(1982).
RN [6]
RP CHARACTERIZATION, AND SEQUENCE REVISION TO 133.
RX MEDLINE=84111559; PubMed=6363409;
RA Chase J.W., L'Italien J.J., Murphy J.B., Spicer E.K., Williams K.R.;
RT "Characterization of the Escherichia coli SSB-113 mutant single-
RT stranded DNA-binding protein. Cloning of the gene, DNA and protein
RT sequence analysis, high pressure liquid chromatography peptide
RT mapping, and DNA-binding studies.";
RL J. Biol. Chem. 259:805-814(1984).
RN [7]
RP MUTANT SSB-1.
RX MEDLINE=85006904; PubMed=6384214;
RA Williams K.R., Murphy J.B., Chase J.W.;
RT "Characterization of the structural and functional defect in the
RT Escherichia coli single-stranded DNA binding protein encoded by the
RT ssb-1 mutant gene. Expression of the ssb-1 gene under lambda pL
RT regulation.";
RL J. Biol. Chem. 259:11804-11811(1984).
RN [8]
RP MUTANT SSB-1, AND DNA-BINDING.
RX MEDLINE=91108818; PubMed=1988680;
RA Bujalowski W., Lohman T.M.;
RT "Monomers of the Escherichia coli SSB-1 mutant protein bind single-
RT stranded DNA.";
RL J. Mol. Biol. 217:63-74(1991).
RN [9]
RP MUTANTS SSB-200; SSB-201 AND SSB-202.
RC STRAIN=K12 / W3110;
RX MEDLINE=97284491; PubMed=9139905;
RA Reddy M., Gowrishankar J.;
RT "Identification and characterization of ssb and uup mutants with
RT increased frequency of precise excision of transposon Tn10
RT derivatives: nucleotide sequence of uup in Escherichia coli.";
RL J. Bacteriol. 179:2892-2899(1997).
RN [10]
RP MUTAGENESIS, AND DNA-BINDING.
RX MEDLINE=87276593; PubMed=3301414; DOI=10.1016/0014-5793(87)80844-X;
RA Casas-Finet J.R., Khamis M.I., Maki A.W., Chase J.W.;
RT "Tryptophan 54 and phenylalanine 60 are involved synergistically in
RT the binding of E. coli SSB protein to single-stranded
RT polynucleotides.";
RL FEBS Lett. 220:347-352(1987).
RN [11]
RP REVIEW.
RX MEDLINE=91203755; PubMed=2087220;
RA Meyer R.R., Laine P.S.;
RT "The single-stranded DNA-binding protein of Escherichia coli.";
RL Microbiol. Rev. 54:342-380(1990).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-135.
RX MEDLINE=97338073; PubMed=9192620; DOI=10.1073/pnas.94.13.6652;
RA Raghunathan S., Ricard C.S., Lohman T.M., Waksman G.;
RT "Crystal structure of the homo-tetrameric DNA binding domain of
RT Escherichia coli single-stranded DNA-binding protein determined by
RT multiwavelength X-ray diffraction on the selenomethionyl protein at
RT 2.9-A resolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:6652-6657(1997).
RN [13]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1-135.
RX MEDLINE=20392541; PubMed=10932248; DOI=10.1038/77943;
RA Raghunathan S., Kozlov A.G., Lohman T.M., Waksman G.;
RT "Structure of the DNA binding domain of E. coli SSB bound to ssDNA.";
RL Nat. Struct. Biol. 7:648-652(2000).
Feature:
INIT_MET 0 0
CHAIN 1 177 Single-stranded DNA-binding protein.
/FTId=PRO_0000096036.
DOMAIN 5 110 SSB.
DNA_BIND 54 60
MUTAGEN 4 4 G->D: Increased frequency of precise
excision of transposon Tn10 derivatives
(mutant SSB-200).
MUTAGEN 10 10 L->F: Increased frequency of precise
excision of transposon Tn10 derivatives
(mutant SSB-202).
MUTAGEN 24 24 P->S: Increased frequency of precise
excision of transposon Tn10 derivatives
(mutant SSB-202).
MUTAGEN 55 55 H->L: Reduces DNA-binding affinity.
MUTAGEN 55 55 H->Y: Destabilizes the tetramer (mutant
SSB-1).
MUTAGEN 60 60 F->A: Reduces DNA-binding affinity.
MUTAGEN 102 102 V->M: Increased frequency of precise
excision of transposon Tn10 derivatives
(mutant SSB-201).
CONFLICT 120 120 A -> AQ (in Ref. 2).
CONFLICT 133 133 G -> S (in Ref. 1).
CONFLICT 170 170 D -> DG (in Ref. 2).
STRAND 5 16
STRAND 19 21
STRAND 24 26
STRAND 29 36
STRAND 41 41
STRAND 44 47
STRAND 53 60
TURN 61 61
HELIX 62 70
TURN 73 74
STRAND 76 88
STRAND 90 93
STRAND 95 102
TURN 104 105
STRAND 107 110
STRAND 115 115
STRAND 119 123
STRAND 127 128
STRAND 130 131
TURN 132 133
STRAND 135 138
STRAND 140 141
Comments:
-!- FUNCTION: This protein is essential for replication of the
chromosomes and its single-stranded DNA phages. It is also
involved in DNA recombination and repair.
-!- SUBUNIT: Homotetramer.
-!- SIMILARITY: Contains 1 SSB domain.
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Sequence length: 177
ASRGVNKVIL VGNLGQDPEV RYMPNGGAVA NITLATSESW RDKATGEMKE QTEWHRVVLF
GKLAEVASEY LRKGSQVYIE GQLRTRKWTD QSGQDRYTTE VVVNVGGTMQ MLGGRQGGGA
PAGGNIGGGQ PQGGWGQPQQ PQGGNQFSGG AQSRPQQSAP AAPSNEPPMD FDDDIPF