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Description:
Protein umuD (EC 3.4.21.-) [Contains: Protein umuD'].
Molecular weight: 15063
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
01-NOV-1986, sequence version 1.
07-MAR-2006, entry version 5.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UMUD_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M10107 | AAA24728.1 | - |
| EMBL | M13387 | AAA98073.1 | - |
| EMBL | D90752 | BAA36030.1 | - |
| EMBL | D90753 | BAA36038.1 | - |
| EMBL | U00096 | AAC74267.1 | - |
| PIR | A03551 | ZWECD. | |
| PDB | 1AY9 | X-ray | A/B=32-139. |
| PDB | 1I4V | NMR | A/B=26-139. |
| PDB | 1UMU | X-ray | A/B=25-137. |
| MEROPS | S24.003 | -. | |
| ECO2DBASE | A015.1 | 6TH EDITION. | |
| GenomeReviews | U00096_GR | b1183.1 | |
| EchoBASE | EB1050 | -.1 | |
| EcoGene | EG11057 | umuD. | |
| BioCyc | EcoCyc:EG11057-MONOMER | -.1 | |
| LinkHub | P04153 | -.1 |
Keywords:
3D-structure; Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; Hydrolase; Protease; Serine protease; SOS mutagenesis; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=85242679; PubMed=2989817;
RA Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T.;
RT "Structural analysis of the umu operon required for inducible
RT mutagenesis in Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:4336-4340(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=85242678; PubMed=2989816;
RA Perry K.L., Elledge S.J., Mitchell B.B., Marsh L., Walker G.C.;
RT "umuDC and mucAB operons whose products are required for UV light- and
RT chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share
RT homology.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:4331-4335(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97061202; PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
RA Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
RA Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
RA Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
RA Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
RA Yano M., Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP MUTAGENESIS.
RX MEDLINE=92318898; PubMed=1320188; DOI=10.1007/BF00265442;
RA Koch W.H., Ennis D.G., Levine A.S., Woodgate R.;
RT "Escherichia coli umuDC mutants: DNA sequence alterations and UmuD
RT cleavage.";
RL Mol. Gen. Genet. 233:443-448(1992).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF UMUD'.
RX MEDLINE=96202734; PubMed=8614470; DOI=10.1038/380727a0;
RA Peat T.S., Frank E., McDonald J.P., Levine A.S., Woodgate R.,
RA Hendrickson W.A.;
RT "Structure of the UmuD' protein and its regulation in response to DNA
RT damage.";
RL Nature 380:727-730(1996).
Feature:
CHAIN 1 139 Protein umuD.
/FTId=PRO_0000041988.
CHAIN 25 139 Protein umuD'.
/FTId=PRO_0000027305.
ACT_SITE 60 60 Involved in auto-cleavage.
ACT_SITE 97 97 Involved in auto-cleavage.
SITE 24 25 Cleavage (auto-).
MUTAGEN 27 27 P->D: In umuD1; non-cleavable.
MUTAGEN 65 65 G->R: In umuD44; non-cleavable.
MUTAGEN 92 92 G->D: In umuD77; non-cleavable.
HELIX 40 44
STRAND 46 46
HELIX 48 50
STRAND 51 55
STRAND 58 59
TURN 60 61
HELIX 62 64
TURN 65 65
TURN 68 69
STRAND 71 75
TURN 76 77
STRAND 78 78
TURN 82 83
STRAND 85 90
TURN 91 92
STRAND 93 100
STRAND 102 104
STRAND 106 108
STRAND 110 111
TURN 112 113
STRAND 114 114
STRAND 117 118
TURN 121 122
STRAND 123 123
STRAND 125 135
Comments:
-!- FUNCTION: Involved in UV protection and mutation. Essential for
induced (or SOS) mutagenesis. May modify the DNA replication
machinery to allow bypass synthesis across a damaged template.
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 139
MLFIKPADLR EIVTFPLFSD LVQCGFPSPA ADYVEQRIDL NQLLIQHPSA TYFVKASGDS
MIDGGISDGD LLIVDSAITA SHGDIVIAAV DGEFTVKKLQ LRPTVQLIPM NSAYSPITIS
SEDTLDVFGV VIHVVKAMR