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Description:
UvrABC system protein A (UvrA protein) (Excinuclease ABC subunit A).
Molecular weight: 10386
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-APR-1988, integrated into UniProtKB/Swiss-Prot.
01-APR-1988, sequence version 1.
07-MAR-2006, entry version 10.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRA_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M13495 | AAA24754.1 | - |
| EMBL | U00006 | AAC43152.1 | - |
| EMBL | U00096 | AAC77028.1 | - |
| EMBL | X01621 | CAA25764.1 | - |
| EMBL | J01721 | AAA24753.1 | - |
| PIR | A23869 | BVECUA. | |
| ECO2DBASE | H124.0 | 6TH EDITION. | |
| GenomeReviews | U00096_GR | b4058.1 | |
| EchoBASE | EB1054 | -.1 | |
| EcoGene | EG11061 | uvrA. | |
| GO | GO:0005515 | F:protein binding | IPI. |
| HAMAP | MF_00205 | - | 1. |
| InterPro | IPR003439 | ABC_transp_like. | |
| InterPro | IPR004602 | UvrA_ABC. | |
| Pfam | PF00005 | ABC_tran | 1. |
| TIGRFAMs | TIGR00630 | uvra | 1. |
| PROSITE | PS00211 | ABC_TRANSPORTER_1 | 2. |
| PROSITE | PS50893 | ABC_TRANSPORTER_2 | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; DNA-binding; Excision nuclease; Metal-binding; Nucleotide-binding; Repeat; SOS response; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=86168204; PubMed=3007478;
RA Husain I., van Houten B., Thomas D.C., Sancar A.;
RT "Sequences of Escherichia coli uvrA gene and protein reveal two
RT potential ATP binding sites.";
RL J. Biol. Chem. 261:4895-4901(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=94089392; PubMed=8265357;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J.,
RA Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the
RT region from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RX MEDLINE=83299251; PubMed=6310514;
RA Backendorf C., Brandsma J.A., Kartasova T., van de Putte P.;
RT "In vivo regulation of the uvrA gene: role of the '-10' and '-35'
RT promoter regions.";
RL Nucleic Acids Res. 11:5795-5810(1983).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
RX MEDLINE=82220077; PubMed=6283374;
RA Sancar A., Sancar G.B., Rupp W.D., Little J.W., Mount D.W.;
RT "LexA protein inhibits transcription of the E. coli uvrA gene in
RT vitro.";
RL Nature 298:96-98(1982).
RN [6]
RP CHARACTERIZATION.
RX MEDLINE=91208117; PubMed=1826851;
RA Myles G.M., Sancar A.;
RT "Isolation and characterization of functional domains of UvrA.";
RL Biochemistry 30:3834-3840(1991).
RN [7]
RP MUTAGENESIS OF CYS-253.
RX MEDLINE=89380205; PubMed=2550431;
RA Navaratnam S., Myles G.M., Strange R.W., Sancar A.;
RT "Evidence from extended X-ray absorption fine structure and site-
RT specific mutagenesis for zinc fingers in UvrA protein of Escherichia
RT coli.";
RL J. Biol. Chem. 264:16067-16071(1989).
Feature:
CHAIN 1 940 UvrABC system protein A.
/FTId=PRO_0000093049.
DOMAIN 310 587 ABC transporter 1.
DOMAIN 607 937 ABC transporter 2.
NP_BIND 31 38 ATP.
ZN_FING 253 280 C4-type.
NP_BIND 640 647 ATP.
ZN_FING 740 766 C4-type.
MUTAGEN 253 253 C->A,H,S: Reduced activity.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. UvrA is an ATPase and a DNA-binding
protein. A damage recognition complex composed of 2 uvrA and 2
uvrB subunits scans DNA for abnormalities. When the presence of a
lesion has been verified by uvrB, the uvrA molecules dissociate
(By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrB during the search for
lesions (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- MISCELLANEOUS: Binds about 2 zinc atoms/molecule.
-!- SIMILARITY: Belongs to the ABC transporter family. UvrA subfamily.
-!- SIMILARITY: Contains 2 ABC transporter domains.
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Sequence length: 940
MDKIEVRGAR THNLKNINLV IPRDKLIVVT GLSGSGKSSL AFDTLYAEGQ RRYVESLSAY
ARQFLSLMEK PDVDHIEGLS PAISIEQKST SHNPRSTVGT ITEIHDYLRL LFARVGEPRC
PDHDVPLAAQ TVSQMVDNVL SQPEGKRLML LAPIIKERKG EHTKTLENLA SQGYIRARID
GEVCDLSDPP KLELQKKHTI EVVVDRFKVR DDLTQRLAES FETALELSGG TAVVADMDDP
KAEELLFSAN FACPICGYSM RELEPRLFSF NNPAGACPTC DGLGVQQYFD PDRVIQNPEL
SLAGGAIRGW DRRNFYYFQM LKSLADHYKF DVEAPWGSLS ANVHKVVLYG SGKENIEFKY
MNDRGDTSIR RHPFEGVLHN MERRYKETES SAVREELAKF ISNRPCASCE GTRLRREARH
VYVENTPLPA ISDMSIGHAM EFFNNLKLAG QRAKIAEKIL KEIGDRLKFL VNVGLNYLTL
SRSAETLSGG EAQRIRLASQ IGAGLVGVMY VLDEPSIGLH QRDNERLLGT LIHLRDLGNT
VIVVEHDEDA IRAADHVIDI GPGAGVHGGE VVAEGPLEAI MAVPESLTGQ YMSGKRKIEV
PKKRVPANPE KVLKLTGARG NNLKDVTLTL PVGLFTCITG VSGSGKSTLI NDTLFPIAQR
QLNGATIAEP APYRDIQGLE HFDKVIDIDQ SPIGRTPRSN PATYTGVFTP VRELFAGVPE
SRARGYTPGR FSFNVRGGRC EACQGDGVIK VEMHFLPDIY VPCDQCKGKR YNRETLEIKY
KGKTIHEVLD MTIEEAREFF DAVPALARKL QTLMDVGLTY IRLGQSATTL SGGEAQRVKL
ARELSKRGTG QTLYILDEPT TGLHFADIQQ LLDVLHKLRD QGNTIVVIEH NLDVIKTADW
IVDLGPEGGS GGGEILVSGT PETVAECEAS HTARFLKPML