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Description:
UvrABC system protein C (Protein uvrC) (Excinuclease ABC subunit C).
Molecular weight: 68188
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-APR-1988, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 1.
07-MAR-2006, entry version 12.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRC_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X03691 | CAA27329.1 | ALT_INIT |
| EMBL | U00096 | AAC74980.1 | ALT_INIT |
| EMBL | D90832 | BAA15733.1 | - |
| EMBL | M24615 | AAA24756.1 | - |
| EMBL | X05398 | CAA28983.1 | - |
| EMBL | M12299 | AAA98753.1 | - |
| PDB | 1KFT | NMR | A=554-610. |
| ECO2DBASE | I065.0 | 6TH EDITION. | |
| GenomeReviews | U00096_GR | b1913.1 | |
| EchoBASE | EB1056 | -.1 | |
| EcoGene | EG11063 | uvrC. | |
| GO | GO:0005515 | F:protein binding | IPI. |
| HAMAP | MF_00203 | - | 1. |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004791 | UvrC_ABC. | |
| InterPro | IPR001162 | UvrC_C. | |
| InterPro | IPR000305 | UvrC_N. | |
| Pfam | PF01541 | GIY-YIG | 1. |
| Pfam | PF00633 | HHH | 2. |
| Pfam | PF02151 | UVR | 1. |
| ProDom | PD005870 | UvrC_C | 1. |
| SMART | SM00465 | GIYc | 1. |
| SMART | SM00278 | HhH1 | 2. |
| TIGRFAMs | TIGR00194 | uvrC | 1. |
| PROSITE | PS50151 | UVR | 1. |
| PROSITE | PS50164 | UVRC_1 | 1. |
| PROSITE | PS50165 | UVRC_2 | 1. |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=84247323; PubMed=6330676;
RA Sancar G.B., Sancar A., Rupp W.D.;
RT "Sequences of the E. coli uvrC gene and protein.";
RL Nucleic Acids Res. 12:4593-4608(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=86176730; PubMed=3515318;
RA Sharma S., Stark T.F., Beattie W.G., Moses R.E.;
RT "Multiple control elements for the uvrC gene unit of Escherichia
RT coli.";
RL Nucleic Acids Res. 14:2301-2318(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
RX MEDLINE=87231005; PubMed=3295776;
RA Moolenaar G.F., van Sluis C.A., Backendorf C., van de Putte P.;
RT "Regulation of the Escherichia coli excision repair gene uvrC. Overlap
RT between the uvrC structural gene and the region coding for a 24 kD
RT protein.";
RL Nucleic Acids Res. 15:4273-4289(1987).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 604-610.
RX MEDLINE=86111778; PubMed=3003065;
RA Gopalakrishnan A.S., Chen Y.-C., Temkin M., Dowhan W.;
RT "Structure and expression of the gene locus encoding the
RT phosphatidylglycerophosphate synthase of Escherichia coli.";
RL J. Biol. Chem. 261:1329-1338(1986).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF ASP-399; ASP-438; ASP-466 AND HIS-538.
RC STRAIN=K12;
RX MEDLINE=92388118; PubMed=1387639;
RA Lin J.-J., Sancar A.;
RT "Active site of (A)BC excinuclease. I. Evidence for 5' incision by
RT UvrC through a catalytic site involving Asp399, Asp438, Asp466, and
RT His538 residues.";
RL J. Biol. Chem. 267:17688-17692(1992).
RN [8]
RP FUNCTION, AND MUTAGENESIS OF ARG-42.
RX MEDLINE=20138260; PubMed=10671556; DOI=10.1074/jbc.275.7.5120;
RA Verhoeven E.E., van Kesteren M., Moolenaar G.F., Visse R., Goosen N.;
RT "Catalytic sites for 3' and 5' incision of Escherichia coli nucleotide
RT excision repair are both located in UvrC.";
RL J. Biol. Chem. 275:5120-5123(2000).
Feature:
CHAIN 1 610 UvrABC system protein C.
/FTId=PRO_0000138300.
DOMAIN 204 239 UVR.
MUTAGEN 42 42 R->A: Defective in 3' incision but not in
5' incision.
MUTAGEN 399 399 D->A,N: Defective in 5' incision but not
in 3' incision.
MUTAGEN 438 438 D->A,N: Defective in 5' incision but not
in 3' incision.
MUTAGEN 466 466 D->A,N: Defective in 5' incision but not
in 3' incision.
MUTAGEN 538 538 H->F,Y: Defective in 5' incision but not
in 3' incision.
CONFLICT 291 291 E -> K (in Ref. 1 and 2).
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. UvrC both incises the 5' and 3' sides
of the lesion. The N-terminal half is responsible for the 3'
incision and the C-terminal half is responsible for the 5'
incision.
-!- SUBUNIT: Interacts with uvrB in an incision complex.
-!- INTERACTION:
P06958:aceE; NbExp=1; IntAct=EBI-559448, EBI-542683;
P36996:cspC; NbExp=1; IntAct=EBI-559448, EBI-547832;
P38504:glnK; NbExp=1; IntAct=EBI-559448, EBI-559503;
P02342:hupA; NbExp=1; IntAct=EBI-559448, EBI-547648;
P00391:lpdA; NbExp=1; IntAct=EBI-559448, EBI-542856;
P07001:pntA; NbExp=1; IntAct=EBI-559448, EBI-559555;
P0A7L0:rplA; NbExp=1; IntAct=EBI-559448, EBI-543771;
P60422:rplB; NbExp=1; IntAct=EBI-559448, EBI-543515;
P60438:rplC; NbExp=1; IntAct=EBI-559448, EBI-542200;
P60723:rplD; NbExp=1; IntAct=EBI-559448, EBI-545597;
P0A7R1:rplI; NbExp=1; IntAct=EBI-559448, EBI-546437;
P02410:rplM; NbExp=1; IntAct=EBI-559448, EBI-543801;
P02411:rplN; NbExp=1; IntAct=EBI-559448, EBI-545605;
P0A7K6:rplS; NbExp=1; IntAct=EBI-559448, EBI-543891;
P0A7L3:rplT; NbExp=1; IntAct=EBI-559448, EBI-544699;
P02422:rplU; NbExp=1; IntAct=EBI-559448, EBI-542445;
P61175:rplV; NbExp=1; IntAct=EBI-559448, EBI-542255;
P68919:rplY; NbExp=1; IntAct=EBI-559448, EBI-553087;
P0A7M2:rpmB; NbExp=1; IntAct=EBI-559448, EBI-543024;
P02349:rpsA; NbExp=1; IntAct=EBI-559448, EBI-546520;
P0A7V0:rpsB; NbExp=1; IntAct=EBI-559448, EBI-543439;
P0A7V3:rpsC; NbExp=1; IntAct=EBI-559448, EBI-544851;
P0A7V8:rpsD; NbExp=1; IntAct=EBI-559448, EBI-543939;
P0A7W1:rpsE; NbExp=1; IntAct=EBI-559448, EBI-543949;
P02358:rpsF; NbExp=1; IntAct=EBI-559448, EBI-543068;
P02359:rpsG; NbExp=1; IntAct=EBI-559448, EBI-543074;
P0A7W7:rpsH; NbExp=1; IntAct=EBI-559448, EBI-543081;
P0A7R5:rpsJ; NbExp=1; IntAct=EBI-559448, EBI-544602;
P0A7R9:rpsK; NbExp=1; IntAct=EBI-559448, EBI-548800;
P0A7S3:rpsL; NbExp=1; IntAct=EBI-559448, EBI-543960;
P0A7S9:rpsM; NbExp=1; IntAct=EBI-559448, EBI-543165;
P02370:rpsN; NbExp=1; IntAct=EBI-559448, EBI-543971;
P02371:rpsO; NbExp=1; IntAct=EBI-559448, EBI-545119;
P0A7T7:rpsR; NbExp=1; IntAct=EBI-559448, EBI-548844;
P0A7U3:rpsS; NbExp=1; IntAct=EBI-559448, EBI-544015;
P0A7U7:rpsT; NbExp=1; IntAct=EBI-559448, EBI-544023;
P07015:sucA; NbExp=1; IntAct=EBI-559448, EBI-543523;
P07016:sucB; NbExp=1; IntAct=EBI-559448, EBI-558621;
P76128:yddS; NbExp=1; IntAct=EBI-559448, EBI-548554;
P77172:yfgF; NbExp=1; IntAct=EBI-559448, EBI-559496;
P07021:yfiB; NbExp=1; IntAct=EBI-559448, EBI-559566;
P33635:yfiF; NbExp=1; IntAct=EBI-559448, EBI-547368;
P42550:yhbY; NbExp=1; IntAct=EBI-559448, EBI-543346;
P31806:yjeF; NbExp=1; IntAct=EBI-559448, EBI-554188;
-!- SUBCELLULAR LOCATION: Cytoplasm.
-!- SIMILARITY: Belongs to the uvrC family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 610
MSDQFDAKAF LKTVTSQPGV YRMYDAGGTV IYVGKAKDLK KRLSSYFRSN LASRKTEALV
AQIQQIDVTV THTETEALLL EHNYIKLYQP RYNVLLRDDK SYPFIFLSGD THPRLAMHRG
AKHAKGEYFG PFPNGYAVRE TLALLQKIFP IRQCENSVYR NRSRPCLQYQ IGRCLGPCVE
GLVSEEEYAQ QVEYVRLFLS GKDDQVLTQL ISRMETASQN LEFEEAARIR DQIQAVRRVT
EKQFVSNTGD DLDVIGVAFD AGMACVHVLF IRQGKVLGSR SYFPKVPGGT ELSEVVETFV
GQFYLQGSQM RTLPGEILLD FNLSDKTLLA DSLSELAGRK INVQTKPRGD RARYLKLART
NAATALTSKL SQQSTVHQRL TALASVLKLP EVKRMECFDI SHTMGEQTVA SCVVFDANGP
LRAEYRRYNI TGITPGDDYA AMNQVLRRRY GKAIDDSKIP DVILIDGGKG QLAQAKNVFA
ELDVSWDKNH PLLLGVAKGA DRKAGLETLF FEPEGEGFSL PPDSPALHVI QHIRDESHDH
AIGGHRKKRA KVKNTSSLET IEGVGPKRRQ MLLKYMGGLQ GLRNASVEEI AKVPGISQGL
AEKIFWSLKH