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Description:
DNA helicase II (EC 3.6.1.-).
Molecular weight: 81990
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
01-APR-1988, sequence version 1.
07-MAR-2006, entry version 62.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRD_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M87049 | AAA67609.1 | - |
| EMBL | U00096 | AAC76816.1 | - |
| EMBL | X00738 | CAA25321.1 | - |
| EMBL | D00069 | BAA00048.1 | - |
| EMBL | X04037 | CAA27671.1 | - |
| EMBL | X00225 | CAA25043.1 | - |
| EMBL | M38257 | AAA24765.1 | - |
| PIR | F65185 | HJECD2. | |
| HSSP | P09980 | 1UAA | |
| IntAct | P03018 | -.1 | |
| GenomeReviews | U00096_GR | b3813.1 | |
| EchoBASE | EB1057 | -.1 | |
| EcoGene | EG11064 | uvrD. | |
| BioCyc | EcoCyc:EG11064-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR005753 | UvrD. | |
| InterPro | IPR000212 | UvrD-helicase. | |
| PANTHER | PTHR11070 | UvrD-helicase.1 | 1. |
| Pfam | PF00580 | UvrD-helicase | 1. |
| TIGRFAMs | TIGR01075 | uvrD | 1. |
Keywords:
ATP-binding; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=92358234; PubMed=1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region
RT from 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX MEDLINE=86304220; PubMed=2943729;
RA Yamamoto Y., Ogawa T., Shinagawa H., Nakayama T., Matsuo H., Ogawa H.;
RT "Determination of the initiation sites of transcription and
RT translation of the uvrD gene of Escherichia coli.";
RL J. Biochem. 99:1579-1590(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=84272253; PubMed=6379604;
RA Finch P.W., Emmerson P.T.;
RT "The nucleotide sequence of the uvrD gene of E. coli.";
RL Nucleic Acids Res. 12:5789-5799(1984).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
RX MEDLINE=84169504; PubMed=6324092;
RA Easton A.M., Kushner S.R.;
RT "Transcription of the uvrD gene of Escherichia coli is controlled by
RT the lexA repressor and by attenuation.";
RL Nucleic Acids Res. 11:8625-8640(1983).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RC STRAIN=K12;
RX MEDLINE=91072248; PubMed=2254268;
RA Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.;
RT "Recombination at ColE1 cer requires the Escherichia coli xerC gene
RT product, a member of the lambda integrase family of site-specific
RT recombinases.";
RL J. Bacteriol. 172:6973-6980(1990).
RN [6]
RP VARIANT UVRD252.
RX MEDLINE=93123151; PubMed=8419285;
RA Washburn B.K., Kushner S.R.;
RT "Characterization of DNA helicase II from a uvrD252 mutant of
RT Escherichia coli.";
RL J. Bacteriol. 175:341-350(1993).
Feature:
CHAIN 1 720 DNA helicase II.
/FTId=PRO_0000102072.
NP_BIND 29 36 ATP (By similarity).
VARIANT 30 30 G -> D (in uvrD252, which is UV
sensitive).
CONFLICT 224 224 D -> N (in Ref. 3).
CONFLICT 291 293 SAA -> NAR (in Ref. 3).
CONFLICT 330 330 A -> T (in Ref. 3).
CONFLICT 540 540 L -> V (in Ref. 3).
Comments:
-!- FUNCTION: Has both ATPase and helicase activities. Unwinds DNA
duplexes with 3' to 5' polarity with respect to the bound strand
and initiates unwinding most effectively when a single-stranded
region is present. Involved in the post-incision events of
nucleotide excision repair and methyl-directed mismatch repair.
-!- INTERACTION:
P23367:mutL; NbExp=2; IntAct=EBI-559573, EBI-554913;
-!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
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Sequence length: 720
MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE NCSPYSIMAV
TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQL
RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA
CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT
GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN
NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC AILYRSNAQS
RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD
RTLDVVRQTS RDRQLTLWQA CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ
TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL
EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG
TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV AFQGQGIKWL VAAYARLESV