|
|
|
|
|
|
Description:
Very short patch repair protein (EC 3.1.-.-) (DNA mismatchendonuclease) (Vsr mismatch endonuclease) (V.EcoKDcm).
Molecular weight: 17884
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) mismatch repair( GO:0006298 )
Important dates:
01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
30-MAY-2000, sequence version 2.
07-MAR-2006, entry version 58.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein VSR_ECOLI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X13330 | CAA31707.1 | - |
| EMBL | M32307 | AAA03724.1 | - |
| EMBL | U00096 | AAC75026.1 | - |
| EMBL | D90835 | BAA15787.1 | - |
| PIR | JS0264 | JS0264. | |
| PDB | 1CW0 | X-ray | A=1-155. |
| PDB | 1ODG | X-ray | A=22-155. |
| PDB | 1VSR | X-ray | A=20-155. |
| REBASE | 3641 | V.EcoKDcm | |
| GenomeReviews | U00096_GR | b1960.1 | |
| EchoBASE | EB1061 | -.1 | |
| EcoGene | EG11068 | vsr. | |
| BioCyc | EcoCyc:EG11068-MONOMER | -.1 | |
| InterPro | IPR004603 | Vsr. | |
| Pfam | PF03852 | Vsr | 1. |
| PIRSF | PIRSF018267 | VSR_endonuc | 1. |
| ProDom | PD016088 | Vsr | 1. |
| TIGRFAMs | TIGR00632 | vsr | 1. |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=89345179; PubMed=2527357;
RA Hanck T., Gerwin N., Fritz H.-J.;
RT "Nucleotide sequence of the dcm locus of Escherichia coli K12.";
RL Nucleic Acids Res. 17:5844-5844(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=90330522; PubMed=2198248;
RA Sohail A., Lieb M., Dar M., Bhagwat A.S.;
RT "A gene required for very short patch repair in Escherichia coli is
RT adjacent to the DNA cytosine methylase gene.";
RL J. Bacteriol. 172:4214-4221(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA Yamamoto Y., Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [5]
RP CHARACTERIZATION.
RX MEDLINE=92049708; PubMed=1944537; DOI=10.1038/353776a0;
RA Hennecke F., Kolmar H., Bruendi K., Fritz H.-J.;
RT "The vsr gene product of E. coli K-12 is a strand- and sequence-
RT specific DNA mismatch endonuclease.";
RL Nature 353:776-778(1991).
RN [6]
RP CHARACTERIZATION.
RX MEDLINE=20330377; PubMed=10871403; DOI=10.1093/nar/28.13.2535;
RA Fox K.R., Allinson S.L., Sahagun-Krause H., Brown T.;
RT "Recognition of GT mismatches by Vsr mismatch endonuclease.";
RL Nucleic Acids Res. 28:2535-2540(2000).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX MEDLINE=20077644; PubMed=10612397; DOI=10.1016/S0092-8674(00)81550-0;
RA Tsutakawa S.E., Jingami H., Morikawa K.;
RT "Recognition of a TG mismatch: the crystal structure of very short
RT patch repair endonuclease in complex with a DNA duplex.";
RL Cell 99:615-623(1999).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 21-156.
RX MEDLINE=99288450; PubMed=10360178; DOI=10.1016/S1097-2765(00)80355-X;
RA Tsutakawa S.E., Muto T., Kawate T., Jingami H., Kunishima N.,
RA Ariyoshi M., Kohda D., Nakagawa M., Morikawa K.;
RT "Crystallographic and functional studies of very short patch repair
RT endonuclease.";
RL Mol. Cell 3:621-628(1999).
Feature:
INIT_MET 0 0
CHAIN 1 155 Very short patch repair protein.
/FTId=PRO_0000200289.
METAL 50 50 Magnesium 1.
METAL 50 50 Magnesium 2.
METAL 62 62 Magnesium 1.
STRAND 3 4
HELIX 6 14
TURN 15 15
STRAND 18 20
HELIX 25 33
TURN 34 35
STRAND 39 41
TURN 43 44
STRAND 45 45
TURN 46 47
STRAND 50 53
HELIX 54 56
TURN 57 57
STRAND 58 63
TURN 65 69
STRAND 71 72
TURN 73 74
STRAND 79 81
HELIX 82 105
TURN 106 107
STRAND 109 114
HELIX 115 118
STRAND 119 120
TURN 121 122
HELIX 126 138
TURN 139 140
STRAND 143 147
TURN 148 149
STRAND 150 153
Comments:
-!- FUNCTION: Deamination of 5-methylcytosine in DNA results in T/G
mismatches. If unrepaired, these mismatches can lead to C-to-C
transition mutations. The very short patch (VSP) repair process in
E.coli counteracts the mutagenic process by repairing the
mismatches in favor of the G-containing strand. This enzyme is an
endonuclease that nicks double-stranded DNA within the sequence
CT(AT)GN or NT(AT)GG next to the thymidine residue that is
mismatched to 2'-deoxyguanosine. The incision is mismatch-
dependent and strand-specific.
-!- COFACTOR: Binds 2 magnesium ions per subunit.
-!- COFACTOR: Binds 1 zinc ion per subunit.
-!- SIMILARITY: Belongs to the vsr family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 155
ADVHDKATRS KNMRAIATRD TAIEKRLASL LTGQGLAFRV QDASLPGRPD FVVDEYRCVI
FTHGCFWHHH HCYLFKVPAT RTEFWLEKIG KNVERDRRDI SRLQELGWRV LIVWECALRG
REKLTDEALT ERLEEWICGE GASAQIDTQG IHLLA