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Description:
N-glycosylase/DNA lyase (AGOG) (8-oxoguanine DNA glycosylase)(EC 3.2.2.-) (DNA-(apurinic or apyrimidinic site) lyase)(EC 4.2.99.18) (AP lyase).
Molecular weight: 31756
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
01-NOV-1999, sequence version 1.
07-MAR-2006, entry version 20.
Phylogenetic order:
Archaea Crenarchaeota Thermoprotei Desulfurococcales Desulfurococcaceae Aeropyrum.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein AGOG_AERPE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000002 | BAA79686.1 | - |
| PIR | F72660 | F72660. | |
| GenomeReviews | BA000002_GR | APE0710.1 | |
| BioCyc | APER56636:APE0710-MONOMER | -.1 | |
| HAMAP | MF_01168 | - | 1. |
Keywords:
Complete proteome; DNA damage; DNA excision; DNA repair; Hydrolase; Lyase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K1;
RX MEDLINE=99310339; PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y.,
RA Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H.,
RA Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H.,
RA Takamiya M., Masuda S., Funahashi T., Tanaka T., Kudoh Y.,
RA Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y.,
RA Nomura N., Sako Y., Kikuchi H.;
RT "Complete genome sequence of an aerobic hyper-thermophilic
RT crenarchaeon, Aeropyrum pernix K1.";
RL DNA Res. 6:83-101(1999).
Feature:
CHAIN 1 291 N-glycosylase/DNA lyase.
/FTId=PRO_0000185108.
REGION 137 206 Helix-hairpin-helix.
ACT_SITE 163 163 Schiff-base intermediate with DNA (By
similarity).
ACT_SITE 194 194 Potential.
BINDING 38 38 8-oxoguanine (By similarity).
BINDING 65 65 8-oxoguanine; via carbonyl oxygen (By
similarity).
BINDING 76 76 8-oxoguanine (By similarity).
BINDING 167 167 8-oxoguanine (By similarity).
BINDING 192 192 8-oxoguanine; via carbonyl oxygen (By
similarity).
BINDING 241 241 8-oxoguanine (By similarity).
BINDING 245 245 8-oxoguanine (By similarity).
Comments:
-!- FUNCTION: DNA repair enzyme that is part of the base excision
repair (BER) pathway; protects from oxidative damage by removing
the major product of DNA oxidation, 8-oxoguanine (GO), from
single- and double-stranded DNA substrates (By similarity).
-!- CATALYTIC ACTIVITY: Cleaves the N-glycosidic bond between the
aberrant base and the sugar-phosphate backbone to generate an
apurinic (AP) site. Cleaves the phosphodiester bond 3' from the AP
site by an elimination reaction, leaving a 3'-terminal unsaturated
sugar and a product with a terminal 5'-phosphate.
-!- DOMAIN: Contains two alpha-helical subdomains, with the 8-
oxoguanine binding site located in a cleft at their interface.
Contains a helix-hairpin-helix (HhH) structural motif and a
Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
-!- SIMILARITY: Belongs to the archaeal N-glycosylase/DNA lyase (AGOG)
family.
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Sequence length: 291
MSLLAQRVRW ERVERVAEAF SRLSIGEVLG FEEQVDPQYK LVSRLAGEIG AGKAALSALL
VGLASYRLAM RGEEWWLCFY RHMRSSLPRA EGLRGVLRAV EGFLTSCSGA AIGREAKLRR
VRRAASAAEV LGEVLDNPLV LVERPSEVLE ALRVALGEKG FRKTTVFSVK IAYYAVRPLA
GRKPLTLDVP IPVDVRVACA SISSEMVEAP SYREVVARPE AAQRAWGRVA RSSGIPVLHI
DSILWVTGWA PRELPPGEAR EAVAGILSRA LDRGKAVLLA SELVRRPCPG G