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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 76207
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
01-MAR-2002, sequence version 1.
07-MAR-2006, entry version 30.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Salmonella.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_SALTI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL627268 | CAD05246.1 | - |
| EMBL | AE014613 | AAO69706.1 | - |
| HSSP | P07025 | 1QOJ | |
| SMR | Q8Z889 | 623-673.1 | |
| GenomeReviews | AL513382_GR | STY0831.1 | |
| GenomeReviews | AE014613_GR | t2089.1 | |
| BioCyc | SENT209261:T2089-MONOMER | -.1 | |
| BioCyc | SENT90370:STY0831-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CT18;
RX MEDLINE=21534947; PubMed=11677608; DOI=10.1038/35101607;
RA Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA Baker S., Basham D., Brooks K., Chillingworth T., Connerton P.,
RA Cronin A., Davis P., Davies R.M., Dowd L., White N., Farrar J.,
RA Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K.,
RA Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C.,
RA Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K.,
RA Whitehead S., Barrell B.G.;
RT "Complete genome sequence of a multiple drug resistant Salmonella
RT enterica serovar Typhi CT18.";
RL Nature 413:848-852(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ty2 / ATCC 700931;
RX MEDLINE=22531367; PubMed=12644504;
RX DOI=10.1128/JB.185.7.2330-2337.2003;
RA Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J.,
RA Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2
RT and CT18.";
RL J. Bacteriol. 185:2330-2337(2003).
Feature:
CHAIN 1 673 UvrABC system protein B.
/FTId=PRO_0000138422.
DOMAIN 633 668 UVR.
NP_BIND 39 46 ATP (Potential).
MOTIF 92 115 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 673
MSKPFKLNSA FKPSGDQPDA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA NVIADLQRPT
MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA YVPSSDTFIE KDASVNEHIE
QMRLSATKAL LERRDVVVVA SVSAIYGLGD PDLYLKMMLH LTVGMLIDQR AILRRLAELQ
YTRNDQAFQR GTFRVRGEVI DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQVESTVPR
YTIYPKTHYV TPRERILQAM EEIKDELADR RKVLLANNKL LEEQRLSQRT QFDLEMMNEL
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM YRGDRARKET
LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE LEKSGDEVVD QVVRPTGLLD
PIIEVRPVAT QVDDLLSEIR QRAAINERVL VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI
DTVERMEIIR DLRLGEFDVL VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG
RAARNVNGKA ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG
QNIAKTKAKG KGKGRSTAKA GIVELDMTPK ALQQKIHELE EQMMQHAQNL EFEEAAQIRD
QLHQLRELFI AAS