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Description:
Regulatory protein ada (Regulatory protein of adaptative response)[Contains: Methylated-DNA--protein-cysteine methyltransferase(EC 2.1.1.63) (O-6-methylguanine-DNA alkyltransferase)].
Molecular weight: 39348
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
23-JAN-2002, sequence version 2.
07-MAR-2006, entry version 56.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Salmonella.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ADA_SALTY:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D90221 | BAA14252.1 | ALT_INIT |
| EMBL | AE008801 | AAL21167.1 | - |
| PIR | A39433 | XYEBOT. | |
| HSSP | P06134 | 1SFE | |
| SMR | P26189 | 187-350.1 | |
| GenomeReviews | AE006468_GR | STM2265.1 | |
| StyGene | SG10002 | ada. | |
| BioCyc | STYP99287:STM2265-MONOMER | -.1 | |
| InterPro | IPR004026 | Ada_Zn_bd. | |
| InterPro | IPR000005 | HTHAraC. | |
| InterPro | IPR008332 | MethylG_mtfrase. | |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF02805 | Ada_Zn_binding | 1. |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| Pfam | PF00165 | HTH_AraC | 2. |
| Pfam | PF02870 | Methyltransf_1N | 1. |
| PRINTS | PR00032 | HTHARAC. | |
| SMART | SM00342 | HTH_ARAC | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00041 | HTH_ARAC_FAMILY_1 | 2. |
| PROSITE | PS01124 | HTH_ARAC_FAMILY_2 | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Activator; Complete proteome; DNA damage; DNA repair; DNA-binding; Metal-binding; Methyltransferase; Transcription; Transcription regulation; Transferase; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=91267928; PubMed=1904855;
RA Hakura A., Morimoto K., Sofuni T., Nohmi T.;
RT "Cloning and characterization of the Salmonella typhimurium ada gene,
RT which encodes O6-methylguanine-DNA methyltransferase.";
RL J. Bacteriol. 173:3663-3672(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX MEDLINE=21534948; PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA Waterston R., Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT LT2.";
RL Nature 413:852-856(2001).
Feature:
CHAIN 1 353 Regulatory protein ada.
/FTId=PRO_0000018749.
CHAIN 179 353 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000018750.
DNA_BIND 102 121 H-T-H motif (By similarity).
ACT_SITE 69 69 Acceptor for methyl from phosphotriester
(By similarity).
ACT_SITE 321 321 Alkyl group acceptor (By similarity).
METAL 38 38 Zinc (By similarity).
METAL 42 42 Zinc (By similarity).
METAL 69 69 Zinc (By similarity).
METAL 72 72 Zinc (By similarity).
SITE 128 129 Cleavage (By similarity).
SITE 178 179 Cleavage (By similarity).
Comments:
-!- FUNCTION: Repair of alkylated guanine in DNA by stoichiometrically
transferring the alkyl group at the O-6 position to a cysteine
residue in the enzyme. This is a suicide reaction: the enzyme is
irreversibly inactivated. Can also repair O-4-methylthymine.
-!- FUNCTION: The methylated ADA protein acts as a positive regulator
of its own synthesis, as well as that of other proteins. The
transcription-activating function of the ADA protein resides in
its N-terminus. It activates the transcription of alkA, alkB and
aidB.
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
-!- SIMILARITY: In the C-terminal section; belongs to the MGMT family.
-!- SIMILARITY: Contains 1 HTH araC/xylS-type DNA-binding domain.
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Sequence length: 353
MMKKALLIDD ECWLRVQARD ASADGRFVFA VRTTGVFCRP SCRSKRALRK NVRFFANAQQ
ALDAGFRPCK RCQPDNARAQ QRRLDKIACA CRLLEQETPV TLAFLAQAVA MSPFHLHRLF
KASTGMTPKG WQQAWRARRL REALAKGEPI TAAIYRAGFP DSSSYYRHAD QTLGMTAKQF
RKGGDNVSVR YALTDWVYGR CLVAESERGI CAILPGDSDD ALLAELHTLF PSARHEPADA
LFQQRVRQVV AAINTRDVLL SLPLDIQGTA FQQQVWQALC AIPCGETVSY QQLAATIGKP
TAVRAVASAC GANKLAMVIP CHRVVRRDGA LSGYRWGVRR KAQLLKREAQ KEE