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Description:
DNA polymerase I (EC 2.7.7.7) (POL I).
Molecular weight: 10312
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
23-JAN-2002, sequence version 2.
07-MAR-2006, entry version 42.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Salmonella.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO1_SALTY:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF071212 | AAG43170.1 | - |
| EMBL | AE008886 | AAL22838.1 | - |
| HSSP | P00582 | 1KLN | |
| SMR | Q9F173 | 325-928.1 | |
| GenomeReviews | AE006468_GR | STM3999.1 | |
| StyGene | SG????? | polA. | |
| BioCyc | STYP99287:STM3999-MONOMER | -.1 | |
| InterPro | IPR002562 | 3_5_exonuclease. | |
| InterPro | IPR002421 | 5_3_exonuclease. | |
| InterPro | IPR001098 | DNA_pol. | |
| InterPro | IPR002298 | DNA_polI. | |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR012337 | RNaseH_fold. | |
| Pfam | PF01612 | 3_5_exonuc | 1. |
| Pfam | PF01367 | 5_3_exonuc | 1. |
| Pfam | PF02739 | 5_3_exonuc_N | 1. |
| Pfam | PF00476 | DNA_pol_A | 1. |
| PRINTS | PR00868 | DNAPOLI. | |
| SMART | SM00474 | 35EXOc | 1. |
| SMART | SM00475 | 53EXOc | 1. |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00482 | POLAc | 1. |
| TIGRFAMs | TIGR00593 | pola | 1. |
| PROSITE | PS00447 | DNA_POLYMERASE_A | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RA Huang Y.P., Ito J.;
RT "DNA polymerase I sequence from Salmonella typhimurium.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX MEDLINE=21534948; PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA Waterston R., Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT LT2.";
RL Nature 413:852-856(2001).
Feature:
CHAIN 1 928 DNA polymerase I.
/FTId=PRO_0000101252.
DOMAIN 1 323 5'-3' exonuclease.
DOMAIN 324 517 3'-5' exonuclease.
REGION 324 928 Klenow fragment.
REGION 521 928 Polymerase.
CONFLICT 751 751 G -> R (in Ref. 1).
CONFLICT 848 848 M -> T (in Ref. 1).
CONFLICT 867 867 L -> I (in Ref. 1).
Comments:
-!- FUNCTION: In addition to polymerase activity, this DNA polymerase
exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to
utilize nicked circular duplex DNA as a template and can unwind
the parental DNA strand from its template.
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBUNIT: Single-chain monomer with multiple functions.
-!- SIMILARITY: Belongs to the DNA polymerase type-A family.
-!- SIMILARITY: Contains 1 3'-5' exonuclease domain.
-!- SIMILARITY: Contains 1 5'-3' exonuclease domain.
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Sequence length: 928
MVQIPENPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI MQYQPTHAAV
VFDAKGKTFR DELFEHYKSH RPPMPDDLRA QIEPLHAMVK AMGLPLLAVS GVEADDVIGT
LAREAEKVGR PVLISTGDKD MAQLVTPNIT LINTMTNTIL GPDEVVNKYG VPPELIIDFL
ALMGDSSDNI PGVPGVGEKT AQALLQGLGG LDTLYAEPEK IAGLTFRGAK TMAGKLAQNK
DVAYLSYKLA TIKTDVELEL TCEQLEVQQP IADELLGLFK KYEFKRWTAD VESGKWLQAK
GAKPAAKPQE TVVIDESPSE PAAALSYENY VTILDDVTLE SWIEKLKKAP VFAFDTETDS
LDNIAANLVG LSFAIEPGVA AYVPVAHDYL DAPDQISRQR ALELLKPLLE DEKVRKVGQN
LKYDRGVLQN YGIELRGIAF DTMLESYILN SVAGRHDMDS LSDRWLKHKT ITFEDIAGKG
KNQLTFNQIA LEEAGRYAAE DADVTLQLHL KMWPELQQHK GPLNVFENIE MPLVPVLSRV
ERNGVKIDPA VLHKHSEEIT LRLAELEKKA HDIAGEAFNL SSTKQLQTIL FEKQGIKPLK
KTPGGAPSTS EEVLEELALD YPLPKVILEY RGLAKLKSTY TDKLPLMINP KTGRVHTSYH
QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYL IVSADYSQIE LRIMAHLSRD
KGLLTAFAEG KDIHRATAAE VFGLPLDSVT GEQRRSAKAI NFGLIYGMSA FGLSRQLNIP
RKEAQKYMDL YFERYPGVLE YMERTRAQAK EQGYVETLEG RRLYLPDIKS SNAARRAGAE
RAAINAPMQG TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDLDAVAKRI
HQLMENCTRI DVPLLVEVGS GENWDQAH