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Description:
Exodeoxyribonuclease III (EC 3.1.11.2) (Exonuclease III) (EXO III) (APendonuclease VI).
Molecular weight: 30785
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
01-MAR-2005, sequence version 1.
07-MAR-2006, entry version 8.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Salmonella.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EX3_SALTY:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF108767 | AAD16979.1 | - |
| EMBL | AE008756 | AAL20227.1 | - |
| HSSP | P09030 | 1AKO | |
| SMR | P0A1A9 | 1-267.1 | |
| GenomeReviews | AE006468_GR | STM1302.1 | |
| StyGene | SG10736 | xthA. | |
| InterPro | IPR000097 | APendonclse1. | |
| InterPro | IPR005135 | Exo_endo_phos. | |
| InterPro | IPR004808 | ExoIII_xth. | |
| Pfam | PF03372 | Exo_endo_phos | 1. |
| TIGRFAMs | TIGR00195 | exoDNase_III | 1. |
| TIGRFAMs | TIGR00633 | xth | 1. |
| PROSITE | PS00726 | AP_NUCLEASE_F1_1 | 1. |
| PROSITE | PS00727 | AP_NUCLEASE_F1_2 | 1. |
| PROSITE | PS00728 | AP_NUCLEASE_F1_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Exonuclease; Hydrolase; Magnesium; Metal-binding; Multifunctional enzyme; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX MEDLINE=99173922; PubMed=10074092;
RA Lu C.D., Abdelal A.T.;
RT "Role of ArgR in activation of the ast operon, encoding enzymes of the
RT arginine succinyltransferase pathway in Salmonella typhimurium.";
RL J. Bacteriol. 181:1934-1938(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX MEDLINE=21534948; PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA Waterston R., Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT LT2.";
RL Nature 413:852-856(2001).
Feature:
CHAIN 1 268 Exodeoxyribonuclease III.
/FTId=PRO_0000200024.
ACT_SITE 259 259 Proton acceptor (By similarity).
METAL 34 34 Magnesium or manganese (By similarity).
SITE 153 153 Important for substrate recognition (By
similarity).
Comments:
-!- FUNCTION: Major apurinic-apyrimidinic endonuclease of E.coli. It
removes the damaged DNA at cytosines and guanines by cleaving on
the 3' side of the AP site by a beta-elimination reaction. It
exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair
diesterase and ribonuclease H activities (By similarity).
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage in the 3'- to 5'-
direction to yield nucleoside 5'-phosphates.
-!- SUBUNIT: Monomer (By similarity).
-!- SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family.
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Sequence length: 268
MKFVSFNING LRARPHQLEA IVEKHQPDVI GLQETKVHDE MFPLEEVAKL GYNVFYHGQK
GHYGVALLTK ATPISVRRGF PDDGEEAQRR IIMAEIPSPL GNITVINGYF PQGESRDHPL
KFPAKAQFYQ NLQNYLETEL KCDNPVLIMG DMNISPTDLD IGIGEENRKR WLRTGKCSFL
PEEREWMSRL LKWGLVDTFR QANPQTMDKF SWFDYRSKGF VDNRGLRIDL LLASAPLAER
CAETGIDYDI RSMEKPSDHA PVWATFRV