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Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 19008
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
01-MAR-2005, sequence version 1.
07-MAR-2006, entry version 8.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Salmonella.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_SALTY:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U23465 | AAC43323.1 | - |
| EMBL | AE008773 | AAL20577.1 | - |
| EMBL | U05668 | AAA16267.1 | - |
| HSSP | P16455 | 1QNT | |
| GenomeReviews | AE006468_GR | STM1659.1 | |
| StyGene | SG10262 | ogt. | |
| InterPro | IPR008332 | MethylG_mtfrase. | |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| Pfam | PF02870 | Methyltransf_1N | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=TA1538;
RX MEDLINE=95189728; PubMed=7883708;
RA Yamada M., Sedgwick B., Sofuni T., Nohmi T.;
RT "Construction and characterization of mutants of Salmonella
RT typhimurium deficient in DNA repair of O6-methylguanine.";
RL J. Bacteriol. 177:1511-1519(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX MEDLINE=21534948; PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA Waterston R., Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-171.
RA Lee A.A., Miller C.G., Lombardo M.;
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 171 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139372.
ACT_SITE 139 139 Alkyl group acceptor (Probable).
CONFLICT 104 121 QVMHYGQLAAQLGRPGAA -> RSCTMVNWRRNWDDRAVR
(in Ref. 3).
CONFLICT 146 147 NG -> KR (in Ref. 3).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated.
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 171
MLRLLEEKIA TPLGPLWVVC DEQFRLRAIE WEQYRDRMEQ LLNIHYRHEG YERVSATNPG
GLSDKLADYF AGNLAVIDTL ETATGGTPFQ REVWQALRAI PCGQVMHYGQ LAAQLGRPGA
ARAVGAANGA NPISIVVPCH RVIGRNGTLT GYAGGVQRKE WLLRHEGYLL L