Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31672
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-MAR-2002, sequence version 1.
07-MAR-2006, entry version 34.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Yersinia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_YERPE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AJ414147 | CAC90136.1 | - |
| EMBL | AE013890 | AAM86430.1 | ALT_INIT |
| EMBL | AE017131 | AAS61529.1 | ALT_INIT |
| PIR | AE0159 | AE0159. | |
| HSSP | P12638 | 1QTW | |
| SMR | Q8ZGJ1 | 1-278.1 | |
| GenomeReviews | AE009952_GR | y2879.1 | |
| GenomeReviews | AE017042_GR | YP1286.1 | |
| GenomeReviews | AL590842_GR | YPO1306.1 | |
| BioCyc | YPES187410:Y2879-MONOMER | -.1 | |
| BioCyc | YPES632:YPO1306-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX MEDLINE=21470413; PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G.,
RA Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V.,
RA Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M.,
RA Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM5 / Biovar Mediaevalis;
RX MEDLINE=22137863; PubMed=12142430;
RX DOI=10.1128/JB.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C.,
RA Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V.,
RA Straley S.C., McDonough K.A., Nilles M.L., Matson J.S., Blattner F.R.,
RA Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z.,
RA Jin L., Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J.,
RA Yang H., Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
Feature:
CHAIN 1 285 Probable endonuclease IV.
/FTId=PRO_0000190890.
METAL 69 69 Zinc 1 (By similarity).
METAL 109 109 Zinc 1 (By similarity).
METAL 145 145 Zinc 1 (By similarity).
METAL 145 145 Zinc 2 (By similarity).
METAL 179 179 Zinc 2 (By similarity).
METAL 182 182 Zinc 3 (By similarity).
METAL 216 216 Zinc 2 (By similarity).
METAL 229 229 Zinc 3 (By similarity).
METAL 231 231 Zinc 3 (By similarity).
METAL 261 261 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 285
MKFVGAHVSA AGGVDQAVIR AHELEATAFA LFTKNQRQWR AAPLAEDVIE KFKLACEKYG
YTSAQILPHD SYLINLGHPV TEALEKSREA FIDELVRCQQ LGLSLLNFHP GSHLLQIDED
QCLARIAESI NIALDATEGV TAVIENTAGQ GSNLGFKFEH LAAIIERVED KSRVGVCIDT
CHAFAAGYDL RTEEDCEHTF AALGKIVGFQ YLRGMHLNDA KSEFNSRVDR HHSLGEGNIG
KTVFSYIMRD SRFDNIPLIL ETVNMDIWAE EIAWLKSQAE IEPSL