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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 82318
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-AUG-1998, sequence version 1.
07-MAR-2006, entry version 42.
Phylogenetic order:
Bacteria Aquificae Aquificales Aquificaceae Aquifex.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_AQUAE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000657 | AAC06838.1 | - |
| PIR | D70356 | D70356. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | AE000657_GR | AQ_633.1 | |
| BioCyc | AAEO63363:AQ_633-MONOMER | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00292 | BRCT | 1. |
| SMART | SM00278 | HhH1 | 2. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | 1. |
| PROSITE | PS01055 | DNA_LIGASE_N1 | 1. |
| PROSITE | PS01056 | DNA_LIGASE_N2 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX MEDLINE=98196666; PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex
RT aeolicus.";
RL Nature 392:353-358(1998).
Feature:
CHAIN 1 720 DNA ligase.
/FTId=PRO_0000161735.
DOMAIN 619 709 BRCT.
ACT_SITE 142 142 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA (By similarity).
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
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Sequence length: 720
MFTPEREKEL QEKTRELLRK IKDVKVLSFE EAKKLAEDLR EVIRYHDYKY YVEANPVIPD
YDYDRLFRAL KEIEKKYPEL ITPDSPTQRV ASEISGEFPT VKHYTPMLSL DNAYSEDELR
EFDRRVRQIT GLEVVEYAVE PKLDGAGIAL VYENDLFVRG ATRGDGEYGE DITNNLKTIK
TIPLKAEFSR FGIKLAEIRG EVVIRKDEFQ KLNKERMEEG LPPFANPRNA AAGSIRQKDP
KEVAKRNLEA IVYHLSYVEP PETEPPTHYE SLKMLHTLGF KTLFKDTKVC KGIDEVIEYC
KEWEKKRDSY PYEIDGMVVK VNDRRLWKVL GYTSHHPRWA IAYKFKPRRA VTKLVDVVFQ
VGRTGTITPV GKLEPVELGG VTVSSVSLFN EDFIREKDIR IGDWVVVERA GDVIPYVVEV
LKEKRTGEEK PVEFPKYCPS CGSELVKLPE EVAIRCINIS CPAQSVLRIK HWASRDAMDI
RGLGDATIKL LFNRGLAKDV GDLYYLKLTD ILKLPGFGEK SAMNLLKAIE ESKNRPLDRV
LYGLGIRYVG QTTAKKIAEI INSVWDLKDI PLEKLMRLEG IGYKVARSIK EFFNIPQNLE
VLKKLEKAGV NLAKKVKEKV ADVLKGKTFV FTGTLDCCSR EKAGEIVEML GGKFSNSVTS
KTDYLVVGKD PGATKLSKAK KYGVKTITEE EFVNMIKDYV DLEKIKKEDK KEKPKIGRLF