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Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 32265
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
01-AUG-1998, sequence version 1.
07-MAR-2006, entry version 40.
Phylogenetic order:
Bacteria Aquificae Aquificales Aquificaceae Aquifex.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_AQUAE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000657 | AAC07514.1 | - |
| PIR | E70440 | E70440. | |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | AE000657_GR | AQ_1629.1 | |
| BioCyc | AAEO63363:AQ_1629-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX MEDLINE=98196666; PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex
RT aeolicus.";
RL Nature 392:353-358(1998).
Feature:
CHAIN 1 282 Probable endonuclease IV.
/FTId=PRO_0000190818.
METAL 66 66 Zinc 1 (By similarity).
METAL 106 106 Zinc 1 (By similarity).
METAL 143 143 Zinc 1 (By similarity).
METAL 143 143 Zinc 2 (By similarity).
METAL 176 176 Zinc 2 (By similarity).
METAL 179 179 Zinc 3 (By similarity).
METAL 213 213 Zinc 2 (By similarity).
METAL 226 226 Zinc 3 (By similarity).
METAL 228 228 Zinc 3 (By similarity).
METAL 258 258 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 282
MALFGAHVSS AGSILKTFKR AKDIGAEVFQ FFLRSPRAWY WKGVDKETKQ AFIEKLKDFK
NPVMVHAPYL LNLASPNEEL REKSVKVFLE ELKFCDEVGI HFYNFHPGTA KGISDEEGLR
NVIKSLEEVF SEYTPKFTTV LLENTAGERG DLGKNFKELK EIMNVFRGIK LGVCLDTCHA
FAYGYEINTK EGFENFKREI EKMVGLESVK AVHANDSKVP LGGRKDRHEH IGKGYIGLEG
FKNLLKDEYF STLPYYIETP KENNMDPVNL SVLREIYQNN DL