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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 77339
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-AUG-1998, sequence version 1.
07-MAR-2006, entry version 42.
Phylogenetic order:
Bacteria Aquificae Aquificales Aquificaceae Aquifex.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_AQUAE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000657 | AAC07664.1 | - |
| PIR | B70460 | B70460. | |
| HSSP | Q56243 | 1C4O | |
| GenomeReviews | AE000657_GR | AQ_1856.1 | |
| BioCyc | AAEO63363:AQ_1856-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX MEDLINE=98196666; PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex
RT aeolicus.";
RL Nature 392:353-358(1998).
Feature:
CHAIN 1 663 UvrABC system protein B.
/FTId=PRO_0000138378.
DOMAIN 624 659 UVR.
NP_BIND 42 49 ATP (Potential).
MOTIF 95 118 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 663
MGVSEQRFKL KTNLKPAGDQ PKAIKKLLEN LRKGVKEQTL LGVTGSGKTF TLANVIAKYN
KPTLVVVHNK ILAAQLYREF KELFPENAVE YFVSYYDYYQ PEAYIPEKDL YIEKDASINE
TLERFRHSAT ISVLERRDVI VVASVSCIYG LGKPEHYENL RIKLQRGIRL NLSKLLRKLV
ELGYQRNDFA IKRATFSVRG DVVEIVPSHT EDYLVRVEFW DDEVERIVLM DALNRHVIKE
VEEYSVFPAS HYIAPRPTVE QALKEIEKDL IERVKWFKSQ GREVEAQRLY QRTMYDIEMI
RELGHCKGIE NYSRYFDGRK PGEPPFTLLD YFPEDFLLII DESHMTIPQL RAMYNGDRSR
KEKLVEYGWR LPSALDNRPL KFEEFLERIN QVIYVSATPG DWELERSEAV VEQIVRPTGL
LDPVVEVRPK MNQLENLVKE IKEVKKRKER ALVLTTTKRL AEEIADYLTE RKIRAKYMHS
ELDAIERAQL IRELREGSID VIVGVNLLRE GLDLPEVSLV AIMDADKEGF LRSYQALIQT
IGRAARNIHG KAILYADRIT KAMEKAIQET QRRRKIQEEF NKKHGITPKS IKKPVKELLA
IEELDYVSVP EELPKGVKSE EDILKKIEKL EKEMWKYAQN WEFEKAAKVR DEIKKLKKLL
GLD