Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 39563
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 35.
Phylogenetic order:
Bacteria Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Neisseria.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_NEIMA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AJ391259 | CAB72023.1 | - |
| EMBL | AL162756 | CAB84889.1 | - |
| PIR | A81861 | A81861. | |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | AL157959_GR | NMA1661.1 | |
| BioCyc | NMEN65699:NMA1661-MONOMER | -.1 | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Z2491 / Serogroup A / Serotype 4A;
RX MEDLINE=20187481; PubMed=10722605;
RX DOI=10.1128/IAI.68.4.2082-2095.2000;
RA Klee S.R., Nassif X., Kusecek B., Merker P., Beretti J.L., Achtman M.,
RA Tinsley C.R.;
RT "Molecular and biological analysis of eight genetic islands that
RT distinguish Neisseria meningitidis from the closely related pathogen
RT Neisseria gonorrhoeae.";
RL Infect. Immun. 68:2082-2095(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z2491 / Serogroup A / Serotype 4A;
RX MEDLINE=20222556; PubMed=10761919; DOI=10.1038/35006655;
RA Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M.,
RA Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T.,
RA Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S.,
RA Jagels K., Leather S., Moule S., Mungall K.L., Quail M.A.,
RA Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Whitehead S., Spratt B.G., Barrell B.G.;
RT "Complete DNA sequence of a serogroup A strain of Neisseria
RT meningitidis Z2491.";
RL Nature 404:502-506(2000).
Feature:
CHAIN 1 352 DNA polymerase IV.
/FTId=PRO_0000173928.
DOMAIN 6 186 UmuC.
ACT_SITE 105 105 By similarity.
METAL 10 10 Magnesium (By similarity).
METAL 104 104 Magnesium (By similarity).
SITE 15 15 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 352
MSSRKIIHID MDAFYASVEL REQPHLKGRP VVVAWEGARS VICAASYEAR QFGLHSAMSV
ATAKRLCPQA VYVPPHFDLY RQVSAQIHAV FRRYTDLIEP LSLDEAYLDV TRNFKNIPYA
SEVAKEIRAA IFAETGLTAS AGIAPNKFLA KIASDWRKPN GQFVLPPHKV MAFLETLPLG
KIPGVGKVTL KKMQSLGMQT AGDLRRFERG ELLNHFGRYG YRLYDLARGT DERPVKAERE
CLQISTEITL PEDLPLEQAA GHLPHLAEDL WRQITRKNVE AQSVTLKLKT YDFRIITRTL
TYSSVLPDCT ALLQAAQMLM ARVPPQTEDA FRLIGIGVGH LVPKNQQQDL WA