Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31702
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 31.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_VIBCH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE004306 | AAF95503.1 | - |
| PIR | D82086 | D82086. | |
| HSSP | P12638 | 1QUM | |
| GenomeReviews | AE003852_GR | VC2360.1 | |
| TIGR | VC2360 | -. | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=El Tor N16961 / Serotype O1;
RX MEDLINE=20406833; PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A.,
RA Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L.,
RA Ermolaeva M.D., Vamathevan J.J., Bass S., Qin H., Dragoi I.,
RA Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D.,
RA Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R.,
RA Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
Feature:
CHAIN 1 286 Probable endonuclease IV.
/FTId=PRO_0000190884.
METAL 71 71 Zinc 1 (By similarity).
METAL 111 111 Zinc 1 (By similarity).
METAL 147 147 Zinc 1 (By similarity).
METAL 147 147 Zinc 2 (By similarity).
METAL 181 181 Zinc 2 (By similarity).
METAL 184 184 Zinc 3 (By similarity).
METAL 218 218 Zinc 2 (By similarity).
METAL 231 231 Zinc 3 (By similarity).
METAL 233 233 Zinc 3 (By similarity).
METAL 263 263 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 286
MYEKLIGAHV SASGGVELAP VRAHEIGANA FALFTKNQRQ WAAKPLEASS IRAFKANCKK
WGFGSEAILP HDSYLINLGA PEPEKLDKSR AAFVDEMLRC DQLGLTLLNF HPGSHLQQVS
EEACLATIAE SINLAHRQVP NVIAVIENTA GQGSNLGWRF EHLAAIIDQV EDKERVGVCL
DTCHTFAAGY DLRTKAACDE TFAEFERVVG MHYLRAMHIN DSKGKLASRV DRHHSLGMGE
IGWECFEYIA QDARFNGIPL ILETIDPDIW ATEIATLRKF STQKEN