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Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 40465
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 22.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_VIBPA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000031 | BAC60605.1 | - |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | BA000031_GR | VP2342.1 | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633 / Serotype O3:K6;
RX MEDLINE=22508454; PubMed=12620739; DOI=10.1016/S0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
Feature:
CHAIN 1 354 DNA polymerase IV.
/FTId=PRO_0000173963.
DOMAIN 8 189 UmuC.
ACT_SITE 108 108 By similarity.
METAL 12 12 Magnesium (By similarity).
METAL 107 107 Magnesium (By similarity).
SITE 17 17 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 354
MSERIRKIIH VDMDCFYAAV EMRDNPNYRD IALAVGGHEK QRGVISTCNY EARKFGVRSA
MPTARALQLC PHLLVVPGRM HIYKQVSLQI RAIFERYTSL IEPLSLDEAY LDVTDATACR
GSATLIAESI RNDIRNELGL TASAGIAPIK FLAKVASDMN KPNGQFVIPP EKVQEVVDKL
PLEKIPGVGK VSLEKLHQAG FYLCEDIKNS DYRELLRQFG RQGASLWKRS HGIDDREVVV
ERERKSVGVE RTFSQNISTY DECWQVIEEK LYPELEKRLE RASPDKSIIK QGIKVKFADF
QLTTIEHIHP QLELEDFKLL LKDILKRQNG REIRLLGLSV MLKPEEQARQ LSFF