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Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 33017
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
23-MAY-2003, sequence version 1.
07-MAR-2006, entry version 24.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_VIBPA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000031 | BAC58761.1 | - |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | BA000031_GR | VP0498.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633 / Serotype O3:K6;
RX MEDLINE=22508454; PubMed=12620739; DOI=10.1016/S0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
Feature:
CHAIN 1 295 Probable endonuclease IV.
/FTId=PRO_0000190885.
METAL 78 78 Zinc 1 (By similarity).
METAL 118 118 Zinc 1 (By similarity).
METAL 154 154 Zinc 1 (By similarity).
METAL 154 154 Zinc 2 (By similarity).
METAL 188 188 Zinc 2 (By similarity).
METAL 191 191 Zinc 3 (By similarity).
METAL 225 225 Zinc 2 (By similarity).
METAL 238 238 Zinc 3 (By similarity).
METAL 240 240 Zinc 3 (By similarity).
METAL 270 270 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 295
MTNMKNKFGN KLIGAHVSAA GGVDQAPLRA REIGANAFAL FTKNQRQWVA KPLEAKTISA
FKANCKMLGF GAEHILPHDS YLINLGAPEA EKLDKSRAAF IDEMERCNQL GLTLLNFHPG
SHLKKVSEQE CLATIAESIN LAHKTVPDVV AVIENTAGQG TNLGWKFEHL AEIIEQVEDK
DRVGVCIDTC HTFTAGYDLR TKEDCERTFA EFDRIVGMHY LRAMHLNDSK VEFASKVDRH
HSLGKGEIGW DCFEYIAKDS RFDGIPLILE TIDPDIWQQE INTLRQFHLA AINNQ