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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 22710
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
23-MAY-2003, sequence version 1.
07-MAR-2006, entry version 22.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_VIBPA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000031 | BAC61208.1 | - |
| HSSP | P03033 | 1JHH | |
| SMR | Q87KN2 | 2-202.1 | |
| MEROPS | S24.001 | -. | |
| GenomeReviews | BA000031_GR | VP2945.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR013324 | Sigma_r3_r4. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633 / Serotype O3:K6;
RX MEDLINE=22508454; PubMed=12620739; DOI=10.1016/S0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
Feature:
CHAIN 1 206 LexA repressor.
/FTId=PRO_0000170102.
DNA_BIND 28 48 H-T-H motif (By similarity).
ACT_SITE 123 123 Involved in auto-cleavage (By
similarity).
ACT_SITE 160 160 Involved in auto-cleavage (By
similarity).
SITE 88 89 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 206
MKPLTPRQQQ VFDLIKSKID DTGMPPTRAE IARELGFRSA NAAEEHLKAL ARKQAIEIIP
GASRGIRILL EDAANDEQGL PLIGQVAAGE PILAQEHVEA HYQVDPAMFK PQADFLLRVN
GESMKDIGIM DGDLLAVHKT QDVRDGQVVV ARVDDDVTVK RLERKGSTVL LHAENEEFAP
IQVDLTSQHL TIEGLAVGII RNTDWM