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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 77070
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
16-JUN-2003, sequence version 1.
07-MAR-2006, entry version 22.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_VIBPA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000031 | BAC60363.1 | - |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | BA000031_GR | VP2100.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633 / Serotype O3:K6;
RX MEDLINE=22508454; PubMed=12620739; DOI=10.1016/S0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
Feature:
CHAIN 1 676 UvrABC system protein B.
/FTId=PRO_0000138444.
DOMAIN 636 671 UVR.
NP_BIND 39 46 ATP (Potential).
MOTIF 92 115 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 676
MSKVYELVSE YQPSGDQPTA IKQLLEGLDA GLAHQTLLGV TGSGKTFTLA NVIAQAQRPA
ILLAPNKTLA AQLYGEMKSF FPNNAVEYFV SYYDYYQPEA YVPTTDTFIE KDASVNAHIE
QMRLSATKAL LERKDAIIVA SVSAIYGLGD PESYLQMMLH LRRGDVIDQR DMLRRLAELQ
YSRNDVAFER GQFRVRGEVI DIFPAESDQD AVRVEMFDDE VDCISVFDPL TGVVKQRDLP
RYTIYPKTHY VTPRDRILEA IESIKVELEV RKKQLLENNK LIEEQRISQR TQFDIEMMNE
LGFCSGIENY SRYLSGRSEG EPPPTLFDYL PHDGLLIIDE SHVTVPQIGA MYKGDRSRKE
TLVEFGFRLP SALDNRPLKF EEFESLAPQT IFVSATPGNY ELEKSAGEIA DQVVRPTGLL
DPILEVRPVA TQVDDLLSEI RIRAAKEERV LVTTLTKRMA EDLTEYLHEH DVRVRYLHSD
IDTVERVEII RDLRLGEFDV LVGINLLREG LDMPEVSLVA ILDADKEGFL RSERSLIQTI
GRAARNIEGK AILYADNITK SMKKAMDETN RRREKQQAYN EKMGITPQAL KRNIKDIMEL
GDITKSKRQR NTKQVPLSKV AEPSQTYEVM SPQQLEKEIS RLEAAMYQHA QDLEFELAAE
KRDEIEKLRA QFIANS