Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 32679
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
01-MAR-2003, sequence version 1.
07-MAR-2006, entry version 22.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_VIBVU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE016795 | AAO09058.1 | - |
| HSSP | P12638 | 1QUM | |
| GenomeReviews | AE016795_GR | VV10541.1 | |
| BioCyc | VVUL216895:VV10541-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CMCP6;
RA Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H.,
RA Choy H.E.;
RT "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 293 Probable endonuclease IV.
/FTId=PRO_0000190886.
METAL 78 78 Zinc 1 (By similarity).
METAL 118 118 Zinc 1 (By similarity).
METAL 154 154 Zinc 1 (By similarity).
METAL 154 154 Zinc 2 (By similarity).
METAL 188 188 Zinc 2 (By similarity).
METAL 191 191 Zinc 3 (By similarity).
METAL 225 225 Zinc 2 (By similarity).
METAL 238 238 Zinc 3 (By similarity).
METAL 240 240 Zinc 3 (By similarity).
METAL 270 270 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 293
MTNSKNRFGN KFIGAHVSAA GGVDNAPLRA REIGANAFAL FTKNQRQWVA KPLEEKTISA
FKANCALLGF SPQQILPHDS YLINLGAPEA EKLEKSRLAF IDEMERCQLL GLNLLNFHPG
SHLEKISEKA CLSLIAESIN LAHQAVPDVV AVIENTAGQG SNLGWRFEHL AEIIEQVEDK
SRVGVCLDTC HTFAAGYDLR TPEACETTFA EFERVVGMHY LRAMHINDSK VKLASKVDRH
HALGKGEIGW DCFEYIAKDS RFDSIPLILE TIEPELWPQE IEQLRKYHLS SIA