Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 22747
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
01-MAR-2003, sequence version 1.
07-MAR-2006, entry version 23.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Alteromonadales Shewanellaceae Shewanella.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_SHEON:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE015892 | AAN57563.1 | - |
| HSSP | P03033 | 1JHH | |
| MEROPS | S24.001 | -. | |
| GenomeReviews | AE014299_GR | SO4603.1 | |
| TIGR | SO4603 | -. | |
| BioCyc | SONE211586:SO4603-MONOMER | -.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR013324 | Sigma_r3_r4. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MR-1;
RX MEDLINE=22297686; PubMed=12368813; DOI=10.1038/nbt749;
RA Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A.,
RA Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J.,
RA Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S.,
RA Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A.,
RA White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M.,
RA Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J.,
RA Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O.,
RA Venter J.C., Nealson K.H., Fraser C.M.;
RT "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT Shewanella oneidensis.";
RL Nat. Biotechnol. 20:1118-1123(2002).
Feature:
CHAIN 1 206 LexA repressor.
/FTId=PRO_0000170084.
DNA_BIND 28 48 H-T-H motif (By similarity).
ACT_SITE 123 123 Involved in auto-cleavage (By
similarity).
ACT_SITE 160 160 Involved in auto-cleavage (By
similarity).
SITE 88 89 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 206
MRPLTPRQAE ILELIKRNIA ETGMPPTRAE IATRLGFKSA NAAEEHLKAL AKKGCIEIMP
GTSRGIRLPV EEEDNSETGL PLIGQVAAGE PILAQEHVEQ YYQIDPSMFH PTANFLLRVK
GDSMKNIGIL EGDLLAVHKV QQARNGQVVV ARVDDDVTVK RFEKKGNVVY LHAENEDYSP
IKVDLSFQSL TIEGLAVGVI RNGDWL