|
|
|
|
|
|
Description:
DNA mismatch repair protein spellchecker 1.
Molecular weight: 10331
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) mismatch repair( GO:0006298 ) postreplication repair( GO:0006301 )
Important dates:
01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
06-DEC-2005, sequence version 4.
07-MAR-2006, entry version 49.
Phylogenetic order:
Eukaryota Metazoa Arthropoda Hexapoda Insecta Pterygota Neoptera Endopterygota Diptera Brachycera Muscomorpha Ephydroidea Drosophilidae Drosophila.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MSH2_DROME:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U17893 | AAA62406.1 | ALT_SEQ |
| EMBL | AE003409 | AAF44872.1 | ALT_SEQ |
| EMBL | AE003643 | AAF53392.2.1 | - |
| EMBL | AE003643 | AAO41195.2.1 | - |
| HSSP | P23909 | 1OH6 | |
| IntAct | P43248 | -.1 | |
| Ensembl | CG4215 | Drosophila melanogaster.1 | |
| FlyBase | FBgn0015546 | spel1. | |
| BioCyc | DMEL-XXX-02:DMEL-XXX-02-008942-MONOMER | -.1 | |
| GO | GO:0005634 | C:nucleus | NAS. |
| GO | GO:0003677 | F:DNA binding | NAS. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0006298 | P:mismatch repair | IMP. |
| GO | GO:0006301 | P:postreplication repair | ISS. |
| PROSITE | PS00486 | DNA_MISMATCH_REPAIR_2 | 1. |
Keywords:
Alternative splicing; ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Canton-S; TISSUE=Head;
RX MEDLINE=99178998; PubMed=10077620; DOI=10.1073/pnas.96.6.2964;
RA Flores C., Engels W.;
RT "Microsatellite instability in Drosophila spellchecker1 (MutS homolog)
RT mutants.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:2964-2969(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX MEDLINE=99403001; PubMed=10471707;
RA Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T.,
RA Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G.,
RA Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G.,
RA Martin C., Moshrefi A.R., Palazzolo M., Reese M.G., Spradling A.C.,
RA Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M.;
RT "An exploration of the sequence of a 2.9-Mb region of the genome of
RT Drosophila melanogaster: the Adh region.";
RL Genetics 153:179-219(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z.,
RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O.,
RA Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RX MEDLINE=22426069; PubMed=12537572;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a
RT systematic review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
Feature:
CHAIN 1 917 DNA mismatch repair protein spellchecker
1.
/FTId=PRO_0000115186.
NP_BIND 667 674 ATP (Potential).
VARSPLIC 736 752 TATDKSLVIIDELGRGT -> VSRETKETKFTYFIQDF
(in isoform C).
/FTId=VSP_016422.
VARSPLIC 753 917 Missing (in isoform C).
/FTId=VSP_016423.
CONFLICT 817 817 S -> P (in Ref. 1).
Comments:
-!- FUNCTION: Involved in postreplication mismatch repair. Binds
specifically to DNA containing mismatched nucleotides thus
providing a target for the excision repair processes
characteristic of postreplication mismatch repair (By similarity).
-!- SUBUNIT: Heterodimer of Msh2/Spel and Msh6 (By similarity).
-!- INTERACTION:
Q9W0M1:CG13889; NbExp=1; IntAct=EBI-233088, EBI-92679;
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=A;
IsoId=P43248-2; Sequence=Displayed;
Note=No experimental confirmation available;
Name=C; Synonyms=B;
IsoId=P43248-1; Sequence=VSP_016422, VSP_016423;
-!- SIMILARITY: Belongs to the DNA mismatch repair mutS family.
-!- CAUTION: Ref.1 and Ref.2 sequences differ from that shown due to
erroneous gene model prediction.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 917
MQAKATDSRQ EPTLNMDTNA RRNFIKFHAK LGEKPATTVR FFDHTDRYTV HGSDDCELVA
KIVYKSTAFI GALLPDDKKE TLQFVSMSKG NFELAVRELL LVRNYRVEVY VKNSSDWEIE
YRGSPGNLLQ FEDILFSNKE VLVGNSIISL LVKLDGGGQR RVGVASVEQN DCKFQLLEFL
DDDFFTELEA TVVLLGPKEC LLPSIEGEYS AVKTLLDRNG VMITMPKKSG DNDLLQDLNR
LLRFAKGQQE DATGLKELQL QLASNALKTA IKYLDLVNDA GNLGHYEIKQ LDLNRFVHLD
SAAVAALNIM PKPGTHPSMP SYRWQSVLGV LDHCRTPQGH RLMGQWVKQP LRSRNILNDR
HNIVQCLLES PDTMETLSLD YLKRIPDILM LTKKLMRRKA NLQDLFRIYQ VILRTPKILK
VLHELDNSTI ESVICAPFKS FLKDLTGLKQ MVEQVVDFEA IERGEYLVKA SFDSRLMELQ
QMMTELYSKM EELQFKCSQE LNLDGKNQVK LESVAKLGHH FRITVKDDSV LRKNKNYRIV
DVIKGGVRFT SDKLEGYADE FASCRTRYEE QQLSIVEEII HVAVGYAAPL TLLNNELAQL
DCLVSFAIAA RSAPTPYVRP KMLEEGAREL VLEDVRHPCL ELQEHVNFIA NSVDFKKEEC
NMFIITGPNM GGKSTYIRSV GTAVLMAHIG AFVPCSLATI SMVDSILGRV GASDNIIKGL
STFMVEMIET SGIIRTATDK SLVIIDELGR GTSTYEGCGI AWSIAEHLAK ETKCFTLFAT
HFHEITKLAE TLSTVKNCHM AAVADADDFT LLYQVRSGVM EKSFGIQVAR LANFPEHVVQ
NAQEVYNEFE DEHVDKQKKE DKALLEKIQV AIQQLSTAGN NVDINVEDLT QLVTQFTKDI
EQLDSDYFKS VLATSEA